| Magnetic barcoded hydrogel microparticles for multiplexed detection. | |
| | |
MedLine Citation:
|
PMID: 20178351 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Magnetic polymer particles have been used in a wide variety of applications ranging from targeting and separation to diagnostics and imaging. Current synthesis methods have limited these particles to spherical or deformations of spherical morphologies. In this paper, we report the use of stop flow lithography to produce magnetic hydrogel microparticles with a graphical code region, a probe region, and a magnetic tail region. These anisotropic multifunctional magnetic polymer particles are an enhanced version of previously synthesized "barcoded" particles (Science, 2007, 315, 1393-1396) developed for the sensitive and rapid multiplexed sensing of nucleic acids. The newly added magnetic region has acquired dipole moments in the presence of weak homogeneous magnetic fields, allowing the particles to align along the applied field direction. The novel magnetic properties have led to practical applications in the efficient orientation and separation of the barcoded microparticles during biological assays without disrupting detection capabilities. |
| | |
Authors:
|
Ki Wan Bong; Stephen C Chapin; Patrick S Doyle |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Langmuir : the ACS journal of surfaces and colloids Volume: 26 ISSN: 1520-5827 ISO Abbreviation: Langmuir Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-26 Completed Date: 2010-09-08 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
|
Languages: eng Pagination: 8008-14 Citation Subset: IM |
Affiliation:
|
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence DNA Probes Hydrogels* Magnetics* Microfluidics Nucleic Acid Hybridization Nucleic Acids / isolation & purification |
| Grant Support | |
ID/Acronym/Agency:
|
R21 EB008814-01A1/EB/NIBIB NIH HHS; R21EB008814/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA Probes; 0/Hydrogels; 0/Nucleic Acids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vacuum Ultraviolet Photofragmentation of Sarcosine: Photoionization Mass Spectrometric and Theoretic...
Next Document: Excess Polarizability Reveals Exciton Localization/Delocalization Controlled by Linking Positions on...