Document Detail


Magnesium transport in Salmonella typhimurium: genetic characterization and cloning of three magnesium transport loci.
MedLine Citation:
PMID:  2548998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella typhimurium strains lacking the CorA Mg2+ transport system retain Mg2+ transport and the ability to grow in medium containing a low concentration of Mg2+. Mutagenesis of a corA strain followed by ampicillin selection allowed isolation of a strain that required Mg2+-supplemented media for growth. This strain contained mutations in at least two loci in addition to corA, designated mgtA and mgtB (for magnesium transport). Strains with mutations at all three loci (corA, mgtA, and mgtB) exhibited no detectable Mg2+ uptake and required 10 mM Mg2+ in the medium for growth at the wild-type rate. A wild-type allele at any one of the three loci was sufficient to restore both Mg2+ transport and growth on 50 microM Mg2+. P22 transduction was used to map the mgt loci. The mgtA mutation was located to approximately 98 map units (cotransducible with pyrB), and mgtB mapped at about 80.5 map units (near gltC). A chromosomal library from S. typhimurium was screened for clones that complemented the Mg2+ requirement of a corA mgtA mgtB mutant. The three classes of plasmids obtained could each independently restore Mg2+ transport to this strain and corresponded to the corA, mgtA, and mgtB loci. Whereas the corA locus of S. typhimurium is analogous to the corA locus previously described for Escherichia coli, neither of the mgt loci described in this report appears analogous to the single mgt locus described in E. coli. Our data in this and the accompanying papers (M. D. Snavely, J. B. Florer, C. G. Miller, and M. E. Maguire, J. Bacteriol. 171:4752-4760, 4761-4766, 1989) indicate that the corA, mgtA, and mgtB loci of S. typhimurium represent three distinct systems that transport Mg2+.
Authors:
S P Hmiel; M D Snavely; J B Florer; M E Maguire; C G Miller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  171     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-09-27     Completed Date:  1989-09-27     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4742-51     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Cloning, Molecular
Crosses, Genetic
DNA Transposable Elements
Genes, Bacterial*
Genotype
Kinetics
Magnesium / metabolism*
Mutation
Plasmids
Restriction Mapping
Salmonella typhimurium / genetics*,  growth & development,  metabolism
Transduction, Genetic
Grant Support
ID/Acronym/Agency:
GM26340/GM/NIGMS NIH HHS; T32-GM07250/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Transposable Elements; 7439-95-4/Magnesium
Comments/Corrections

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