| Magnesium depletion causes growth inhibition, reduced expression of cyclin D1, and increased expression of P27Kip1 in normal but not in transformed mammary epithelial cells. | |
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MedLine Citation:
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PMID: 10395294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, we have evaluated the effects of extracellular magnesium restriction on the growth and cell cycle parameters of normal (HC11) and transformed (MCF-7) breast epithelial cell lines. Cells were incubated in medium with different concentrations of Mg2+ (from 0.5 to 0 mM) and the growth rates were determined by [3H]-thymidine incorporation and cell counting. The growth of the HC11 cells was drastically inhibited by Mg2+ depletion whereas the MCF-7 cells were only slightly inhibited (about 50% and 15%, respectively, after incubation in 0.05 mM Mg for 48 h). Cell cycle analyses showed a decrease in the percentage of cells in the S phase when both cell lines were incubated at low Mg2+ concentration. However, while the percentage of cells in both the G0/G1 and G2/M phases was increased in the HC11 cells, only the percentage of cells in the G2/M phase was increased in the MCF-7 cell line. Extracellular magnesium depletion was associated with increased expression of the cyclin-dependent kinase inhibitor p27Kip1 and decreased expression of cyclin D1 in the HC11 but not in the MCF-7 cells. We also demonstrated that Mg2+ depletion does not inhibit kinase activities in the normal HC11 cells and that Mg2+-restricted HC11 cells are still responsive to the epidermal growth factor (EGF)- and insulin-mediated stimulation of cell growth. These data suggest that normal but not transformed mammary epithelial cells are inhibited by extracellular Mg2+ restriction and that this effect might be mediated by changes in the levels of expression of both cyclin D1 and p27Kip1. |
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Authors:
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A Sgambato; F I Wolf; B Faraglia; A Cittadini |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 180 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1999 Aug |
Date Detail:
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Created Date: 1999-07-16 Completed Date: 1999-07-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 245-54 Citation Subset: IM |
Affiliation:
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Centro di Ricerche Oncologiche Giovanni XXIII, Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, Rome, Italy. ibipg@rm.unicatt.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breast Neoplasms Cell Cycle Proteins* Cell Division / drug effects, physiology Cell Line, Transformed / cytology, drug effects, enzymology Cyclin D1 / genetics* Cyclin E / genetics Cyclin-Dependent Kinase Inhibitor p27 Enzyme Inhibitors / metabolism* Female Flow Cytometry G0 Phase / drug effects Gene Expression Regulation, Neoplastic / drug effects* Humans Magnesium / pharmacology* Mammary Glands, Animal / cytology* Mice Mice, Inbred BALB C Microtubule-Associated Proteins / genetics* Pregnancy S Phase / drug effects Thymidine / metabolism, pharmacology Tritium / diagnostic use Tumor Suppressor Proteins* |
| Chemical | |
Reg. No./Substance:
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0/Cdkn1b protein, mouse; 0/Cell Cycle Proteins; 0/Cyclin E; 0/Enzyme Inhibitors; 0/Microtubule-Associated Proteins; 0/Tumor Suppressor Proteins; 10028-17-8/Tritium; 136601-57-5/Cyclin D1; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; 50-89-5/Thymidine; 7439-95-4/Magnesium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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