Document Detail


Magnesium-dependent association and folding of oligonucleosomes reconstituted with ubiquitinated H2A.
MedLine Citation:
PMID:  11278847     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The MgCl2-induced folding of defined 12-mer nucleosomal arrays, in which ubiquitinated histone H2A (uH2A) replaced H2A, was analyzed by quantitative agarose gel electrophoresis and analytical centrifugation. Both types of analysis showed that uH2A arrays attained a degree of compaction similar to that of control arrays in 2 mM MgCl2. These results indicate that attachment of ubiquitin to H2A has little effect on the ability of nucleosomal arrays to form higher order folded structures in the ionic conditions tested. In contrast, uH2A arrays were found to oligomerize at lower MgCl2 concentrations than control nucleosomal arrays, suggesting that histone ubiquitination may play a role in nucleosomal fiber association.
Authors:
L J Jason; S C Moore; J Ausio; G Lindsey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-02-02
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  276     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-30     Completed Date:  2001-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14597-601     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch 7701, South Africa. laure@molbio.uct.ac.za
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Centrifugation
Chickens
Electrophoresis, Agar Gel
Histones / metabolism*
Magnesium Chloride / metabolism*
Nucleosomes / metabolism*
Ubiquitins / metabolism*
Chemical
Reg. No./Substance:
0/Histones; 0/Nucleosomes; 0/Ubiquitins; 7786-30-3/Magnesium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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