| Magnesium-dependent association and folding of oligonucleosomes reconstituted with ubiquitinated H2A. | |
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MedLine Citation:
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PMID: 11278847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The MgCl2-induced folding of defined 12-mer nucleosomal arrays, in which ubiquitinated histone H2A (uH2A) replaced H2A, was analyzed by quantitative agarose gel electrophoresis and analytical centrifugation. Both types of analysis showed that uH2A arrays attained a degree of compaction similar to that of control arrays in 2 mM MgCl2. These results indicate that attachment of ubiquitin to H2A has little effect on the ability of nucleosomal arrays to form higher order folded structures in the ionic conditions tested. In contrast, uH2A arrays were found to oligomerize at lower MgCl2 concentrations than control nucleosomal arrays, suggesting that histone ubiquitination may play a role in nucleosomal fiber association. |
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Authors:
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L J Jason; S C Moore; J Ausio; G Lindsey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2001-02-02 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 276 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-04-30 Completed Date: 2001-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 14597-601 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch 7701, South Africa. laure@molbio.uct.ac.za |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Centrifugation Chickens Electrophoresis, Agar Gel Histones / metabolism* Magnesium Chloride / metabolism* Nucleosomes / metabolism* Ubiquitins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Histones; 0/Nucleosomes; 0/Ubiquitins; 7786-30-3/Magnesium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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