| MagaZorb: a simple tool for rapid isolation of viral nucleic acids. | |
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MedLine Citation:
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PMID: 20225944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Effective isolation of nucleic acids from samples containing viral materials is an essential step for accurate diagnosis of viral infections. The necessity of this critical step before analytical identification and diagnosis of viral infections is paramount to screening programs and to identifying and monitoring epidemics and pandemics. With molecular assays rapidly evolving into routine practice, clinical laboratories face several challenges, including presence of small amounts of viral nucleic acids in abundant levels of genomic DNA and total RNA, processing of various sample types, and carry-over of polymerase chain reaction inhibitors, which could significantly affect polymerase chain reaction and microarray results. MagaZorb nucleic acid isolation technology overcomes these challenges and offers a simple and reliable method for isolation of high-quality and high-yield nucleic acids. Although the MagaZorb technology is readily adaptable to automated platforms, it is also well suited to laboratories in remote areas of resource-poor countries, because a simple magnet is the only device required to perform the procedure manually. Performance characteristics and clinical application of the MagaZorb technology are briefly described here. |
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Authors:
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Dokhi Nargessi; Chin-Yih Ou |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 201 Suppl 1 ISSN: 1537-6613 ISO Abbreviation: J. Infect. Dis. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-04-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: S37-41 Citation Subset: AIM; IM |
Affiliation:
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Cortex Biochem, San Leandro, California, USA. dnargessi@gmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Automation, Laboratory Developing Countries Humans Magnetics Microbiological Techniques / methods* Molecular Diagnostic Techniques / methods* RNA, Viral / isolation & purification* Virus Diseases / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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