Document Detail

Madin Darby bovine kidney cell synchronization by lovastatin: application to bovine herpesvirus-1 gene expression.
MedLine Citation:
PMID:  7889037     Owner:  NLM     Status:  MEDLINE    
The number of investigations involving cell proliferation has increased rapidly in the last years. One of the major difficulties in studying cell-cycle-related events is obtaining highly synchronous cell populations without metabolic imbalance. This study demonstrates that the Madin Darby bovine kidney (MDBK) cells, a commonly used cell line in veterinary research, can be effectively synchronized using lovastatin (Lov), a drug used to treat hypercholesteremia in humans. This was demonstrated by the following results: (i) Lov inhibits cell proliferation in a dose-dependent manner; (ii) Lov synchronizes MDBK cells mainly in the G1 and secondarily in the G2+M cell-cycle phases; (iii) the cytostatic effect of Lov can be specifically inhibited by addition of mevalonate (Mev) (Lov inhibits the synthesis of Mev); (iv) removal of Lov from G1-arrested cultures, followed by addition of Mev, resulted in the synchronous recovery of DNA synthesis; and (v) 5-bromo2'-deoxyuridine incorporation experiments revealed that MDBK cells synchronization by Lov can be followed for at least 3 cycles after removal of Lov and addition of Mev. Furthermore, as an application of investigations based on the availability of synchronized MDBK, we showed that bovine herpesvirus-1 gene expression is independent on the cell cycle.
A Vanderplasschen; E Hanon; C Benarafa; R Greimers; A Op De Beeck; M Loncar; P P Pastoret
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Veterinary research     Volume:  25     ISSN:  0928-4249     ISO Abbreviation:  Vet. Res.     Publication Date:  1994  
Date Detail:
Created Date:  1995-04-20     Completed Date:  1995-04-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9309551     Medline TA:  Vet Res     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  555-67     Citation Subset:  IM    
Département de Virologie-Immunologie, Faculté de Médecine Vétérinaire, Université de Liège, Belgium.
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MeSH Terms
Cell Cycle / drug effects*
Cell Line / cytology,  drug effects*,  virology*
Flow Cytometry
Gene Expression Regulation, Viral / physiology
Herpesvirus 1, Bovine / genetics*
Kidney / cytology
Lovastatin / pharmacology*
Time Factors
Reg. No./Substance:

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