|Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.|
|PMID: 11707408 Owner: NLM Status: MEDLINE|
|Mad2 is a key component of the spindle checkpoint, a device that controls the fidelity of chromosome segregation in mitosis. The ability of Mad2 to form oligomers in vitro has been correlated with its ability to block the cell cycle upon injection into Xenopus embryos. Here we show that Mad2 forms incompatible complexes with Mad1 and Cdc20, neither of which requires Mad2 oligomerization. A monomeric point mutant of Mad2 can sustain a cell cycle arrest of comparable strength to that of the wild-type protein. We show that the interaction of Mad2 with Mad1 is crucial for the localization of Mad2 to kinetochores, where Mad2 interacts with Cdc20. We propose a model that features the kinetochore as a 'folding factory' for the formation of a Mad2-Cdc20 complex endowed with inhibitory activity on the anaphase promoting complex.|
|L Sironi; M Melixetian; M Faretta; E Prosperini; K Helin; A Musacchio|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: The EMBO journal Volume: 20 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 2001 Nov|
|Created Date: 2001-11-14 Completed Date: 2002-01-17 Revised Date: 2009-11-18|
Medline Journal Info:
|Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England|
|Languages: eng Pagination: 6371-82 Citation Subset: IM|
|Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy.|
|APA/MLA Format Download EndNote Download BibTex|
Antibodies, Monoclonal / metabolism
Calcium-Binding Proteins / metabolism*
Cell Cycle Proteins / metabolism*
DNA, Complementary / metabolism
Electrophoresis, Polyacrylamide Gel
Escherichia coli / metabolism
Fungal Proteins / metabolism*
Kinetochores / metabolism
Peptides / chemistry
Phosphoproteins / metabolism*
Plasmids / metabolism
Polymerase Chain Reaction
Recombinant Proteins / metabolism
Repressor Proteins / metabolism*
Saccharomyces cerevisiae Proteins*
Sodium Chloride / pharmacology
Urea / pharmacology
|0/Antibodies, Monoclonal; 0/CDC20 protein, S cerevisiae; 0/Calcium-Binding Proteins; 0/Carrier Proteins; 0/Cell Cycle Proteins; 0/DNA, Complementary; 0/Fungal Proteins; 0/MAD1 protein, S cerevisiae; 0/MAD1L1 protein, human; 0/MAD2 protein, S cerevisiae; 0/Mad1l1 protein, mouse; 0/Mad2l1 protein, mouse; 0/Mad2l2 protein, mouse; 0/Nuclear Proteins; 0/Peptides; 0/Phosphoproteins; 0/Recombinant Proteins; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins; 57-13-6/Urea; 7647-14-5/Sodium Chloride|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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