| Mad1 suppresses bladder cancer cell proliferation by inhibiting human telomerase reverse transcriptase transcription and telomerase activity. | |
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MedLine Citation:
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PMID: 16765199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To study the effects and possible mechanisms of mitosis arrest deficiency 1 (Mad1), the heterodimerizer of Max and a transcriptional repressor, on cell proliferation of bladder cancer in vitro and in vivo. METHODS: Combining methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, luciferase assay, telomeric repeat amplification protocol-enzyme-linked immunosorbent assay, real-time reverse transcriptase polymerase chain reaction, experimental animal models, and other assays, we detected the alterations of cell proliferation, cell cycle, promoter activity and expression of human telomerase reverse transcriptase (hTERT), and telomerase activity in different treated bladder cells and xenograft tissues. RESULTS: Mad1 inhibited cell proliferation, increased G0/G1 accumulation in cell cycle distribution, decreased the transcription and expression of hTERT, and reduced telomerase activity compared with controls in T24 and EJ cells. Mad1 also arrested tumor growth and downregulated hTERT expression and telomerase activity in bladder cancer xenograft BALB/c nude mice. CONCLUSIONS: Mad1 inhibited the proliferation of human bladder cancer cells by inhibiting hTERT transcription and telomerase activity. Mad1 could be a potentially useful candidate for inhibition of bladder cancer growth. |
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Authors:
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Lin Zou; Penghui Zhang; Chunli Luo; Zhiguang Tu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Urology Volume: 67 ISSN: 1527-9995 ISO Abbreviation: Urology Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-12 Completed Date: 2006-07-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0366151 Medline TA: Urology Country: United States |
Other Details:
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Languages: eng Pagination: 1335-40 Citation Subset: IM |
Affiliation:
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Faculty of Laboratory Medicine at Chongqing University of Medical Sciences, Key Laboratory of Laboratory Medical Diagnosis of Education Ministry, Chongqing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Proteins / genetics*, physiology* Cell Proliferation DNA-Binding Proteins / antagonists & inhibitors* Humans Mice Mice, Inbred BALB C Neoplasm Transplantation Nuclear Proteins / genetics*, physiology* Telomerase / antagonists & inhibitors, metabolism* Transcription, Genetic / physiology* Tumor Cells, Cultured Urinary Bladder Neoplasms / enzymology, genetics*, pathology* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/MAD1L1 protein, human; 0/Nuclear Proteins; EC 2.7.7.49/Telomerase |
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