| Macula densa arginine delivery and uptake in the rat regulates glomerular capillary pressure. Effects of salt intake. | |
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MedLine Citation:
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PMID: 9410901 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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These studies tested the hypothesis that delivery and/or cellular uptake of L-arginine limits macula densa nitric oxide generation and actions on tubuloglomerular feedback (TGF) during salt restriction. Maximal TGF responses were assessed from reductions in proximal stop flow pressure during loop of Henle (LH) perfusion at 40 nl/min with artificial tubular fluid containing vehicles or drugs. Orthograde LH perfusion of L-arginine (10[-3] M) reduced maximal TGF significantly in rats adapted to low salt (LS: 7.9+/-0.4-6.3+/-0.4 mmHg; P < 0.05), but not high salt (HS: 5.8+/-0.3-5.9+/-0.3; NS). The effects were stereospecific and prevented by coperfusion with NG-methyl-L-arginine. Microperfusion of L-arginine (10[-3] M) into the peritubular capillaries reduced the maximum TGF response more in nephrons of LS than HS rats (deltaTGF: LS, 32+/-6 vs. HS, 13+/-4%; P < 0.05) and restored a TGF response to luminal perfusion of NG-methyl-L-arginine in LS rats. Coperfusion of nephrons with excess L-lysine or L-homoarginine, which compete with L-arginine for system y+ transport, blocked the fall in proximal stopflow pressure produced by orthograde LH perfusion of L-arginine in LS rats. Reabsorption of [3H]arginine by the perfused loop segment was similar in LS (93+/-2%) and HS (94+/-1%) rats. Coperfusion with excess L-arginine, L-lysine, or L-homoarginine, however, reduced [3H]arginine reabsorption significantly (P < 0.05) more in HS rats than in LS rats. In conclusion, blunting of maximal TGF responses in salt-restricted rats by nephron-derived NO is limited by L-arginine availability and cellular uptake via system y+. |
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Authors:
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W J Welch; C S Wilcox |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 100 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1997 Nov |
Date Detail:
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Created Date: 1997-12-23 Completed Date: 1997-12-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2235-42 Citation Subset: AIM; IM |
Affiliation:
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Division of Nephrology and Hypertension, Georgetown University Medical Center, Washington, DC 20007, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / metabolism* Blood Pressure Body Weight Capillaries / physiology Homoarginine / metabolism Kidney / anatomy & histology Kidney Glomerulus / blood supply, metabolism* Lysine / metabolism Male Organ Size Rats Rats, Sprague-Dawley Sodium Chloride / metabolism* Water-Electrolyte Balance |
| Grant Support | |
ID/Acronym/Agency:
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DK 36079/DK/NIDDK NIH HHS; DK 49870/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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156-86-5/Homoarginine; 56-87-1/Lysine; 74-79-3/Arginine; 7647-14-5/Sodium Chloride |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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