Document Detail


Macrophages on the nasal mucosal surface in provoked and naturally occurring allergic rhinitis.
MedLine Citation:
PMID:  1759583     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Macrophages are the most common cell type residing in the lumen of the lower airways. However, very little is known about the presence and putative pathogenic implications of macrophages in the upper airways. Using specific immunohistochemical techniques, the presence of and changes in macrophage density were studied before and after allergen exposure in the laboratory and during natural allergen exposure of subjects with seasonal allergic rhinitis. The monoclonal antibody EBM 11 combined with the alkaline phosphatase-anti-alkaline phosphatase-technique was applied on cytospin-prepared slides. In the challenge experiment, 0.5 +/- 0.2% (mean +/- SEM; n = 10) of the total cell number were positive for the EBM 11 marker before challenge, thereby not differing from the controls (0.2 +/- 0.2%; mean +/- SEM; n = 3). Local allergen challenge induced an increase of these cells to a peak of 1.3 +/- 0.4% after 4 h (p less than 0.05). During seasonal exposure there was also a similar increase, from 0.7 +/- 0.2 to 1.3 +/- 0.3% (p less than 0.05; n = 11) in placebo-treated patients and from 0.7 +/- 0.2 to 1.6 +/- 0.4% (p less than 0.05; n = 11) in patients treated with topical glucocorticoids. There was, however, no direct relationship between nasal symptoms and number of macrophages present on the mucosal surface. The study indicates that macrophages are involved in the inflammatory processes of allergic rhinitis.
Authors:
S Juliusson; C Bachert; H Klementsson; G Karlsson; U Pipkorn
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  111     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  1991  
Date Detail:
Created Date:  1992-02-03     Completed Date:  1992-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  946-53     Citation Subset:  IM    
Affiliation:
Department of Oto-Rhino-Laryngology, Sahlgren's Hospital, Gothenburg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Allergens / administration & dosage
Antibodies, Monoclonal / immunology
Antigens, CD / immunology
Antigens, Differentiation, Myelomonocytic / immunology
Bronchial Provocation Tests
Female
Humans
Immunoenzyme Techniques
Leukocyte Count
Macrophages
Male
Nasal Mucosa / immunology*
Rhinitis, Allergic, Seasonal / immunology*
Chemical
Reg. No./Substance:
0/Allergens; 0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human

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