Document Detail


Macrophages in human reproductive tissues contain luteinizing hormone/chorionic gonadotropin receptors.
MedLine Citation:
PMID:  12765348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PROBLEM: To determine whether macrophages in human reproductive tissues contain luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptor mRNA and receptor protein that can bind 125I-hCG. METHOD OF STUDY: Macrophages isolated from term pregnancy human decidua were used for LH/hCG receptor detection by in situ hybridization for receptor mRNA and immunocytochemistry for a macrophage marker, CD68, performed alone and in combination, reverse transcription-nested polymerase chain reaction, Western and ligand blotting. The LH/hCG receptor presence in macrophages in late luteal phase human endometria and corpora lutea was determined by sequential performance of in situ hybridization and immunocytochemistry. RESULTS: The macrophages present in term pregnancy human decidua and late luteal phase human endometria and corpora lutea contain LH/hCG receptors. CONCLUSIONS: This is the first demonstration of macrophages present in human reproductive tissues containing LH/hCG receptors. The receptor presence suggests that LH and hCG may regulate macrophage functions in gonadal as well as in non-gonadal target tissues.
Authors:
Y M Zhang; Ch V Rao; Z M Lei
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  49     ISSN:  1046-7408     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-05-26     Completed Date:  2003-10-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  93-100     Citation Subset:  IM    
Affiliation:
Division of Research, Department of Obstetrics, Gynecology and Women's Health, University of Louisville Health Sciences Center, Louisville, KY 40292, USA.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Decidua / cytology,  metabolism*
Female
Humans
In Situ Hybridization
Ligands
Macrophages / metabolism*
Pregnancy
RNA, Messenger / metabolism
Receptors, LH / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Ligands; 0/RNA, Messenger; 0/Receptors, LH

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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