Document Detail


Macrophages are involved in hexachlorobenzene-induced adverse immune effects.
MedLine Citation:
PMID:  16271622     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hexachlorobenzene (HCB) is a persistent environmental pollutant that causes adverse immune effects in man and rat. The Brown Norway (BN) rat is very susceptible to HCB-induced immunopathology and oral exposure causes inflammatory skin and lung lesions, splenomegaly, lymph node (LN) enlargement, and increased serum levels of IgE and anti-ssDNA IgM. T cells play an important role but do not account for all adverse effects induced by HCB. Macrophages are probably also important and the relationship between macrophages and T cells was further investigated. To eliminate macrophages clodronate-liposomes were used. Furthermore, a kinetic study was performed to obtain insight in the early phase of the HCB-induced immune response. Also, experiments were performed to detect specific memory T cells. Therefore, an adoptive transfer study was performed. Our results indicate that macrophages are indeed involved in HCB-induced skin lesions, lung eosinophilia, and elevation of IgM against ssDNA. Kinetics showed that both skin and lung lesions appeared early after exposure. Moreover, immune effects could not be adaptively transferred. Thus, both macrophages and T cells are involved in HCB-induced immune effects but HCB exposure does not lead to specific T cell sensitization. Presumably, HCB exposure induces macrophage activation, thereby generating adjuvant signals that polyclonally stimulate T cells. Together, these events may lead to the observed immunopathology in BN rats.
Authors:
Janine Ezendam; Kevin Kosterman; Henneke Spijkerboer; Rob Bleumink; Ine Hassing; Nico van Rooijen; Joseph G Vos; Raymond Pieters
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  209     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-07     Completed Date:  2005-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  19-27     Citation Subset:  IM    
Affiliation:
National Institute for Public Health and the Environment, Laboratory for Toxicology, Pathology and Genetics, Bilthoven, PO Box 1 3720 BA, The Netherlands. Janine.Ezendam@rivm.nl
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MeSH Terms
Descriptor/Qualifier:
Analgesics, Non-Narcotic / diagnostic use
Animals
B-Lymphocytes / drug effects
Cell Line
Clodronic Acid / diagnostic use
DNA, Single-Stranded / immunology
Female
Flow Cytometry
Hexachlorobenzene / toxicity*
Immunoglobulin M / analysis
Immunotherapy, Adoptive
Lung / pathology
Lymph Nodes / cytology,  drug effects,  immunology
Lymphocyte Count
Macrophage Activation / drug effects
Macrophages / drug effects,  immunology*
Macrophages, Alveolar / drug effects,  metabolism
Organ Size / drug effects
Rats
Rats, Inbred BN
Skin / pathology
Spleen / cytology,  drug effects,  immunology
T-Lymphocytes / drug effects,  immunology
Weight Gain / drug effects
Chemical
Reg. No./Substance:
0/Analgesics, Non-Narcotic; 0/DNA, Single-Stranded; 0/Immunoglobulin M; 10596-23-3/Clodronic Acid; 118-74-1/Hexachlorobenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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