|Macrophage-stimulating protein activates Ras by both activation and translocation of SOS nucleotide exchange factor.|
|PMID: 7488076 Owner: NLM Status: MEDLINE|
|Macrophage-stimulating protein (MSP) is a chemotactic factor that activates the receptor tyrosine kinase RON. The involvement of Ras in MSP-induced signal transduction was investigated. Here we demonstrate that, in RON-transfected MDCK cells, an active GTP-bound form of Ras was rapidly accumulated by MSP treatment and the Ras-guanine nucleotide exchange activity in SOS immunoprecipitates was concomitantly increased. GAP activity was not changed under the same conditions used. Furthermore, the SH2 domain of adaptor protein GRB2, but not Shc, associated with the activated RON-beta chain, and GRB2-SOS complexes translocated from the cytosol to the membrane upon MSP treatment. These results strongly suggest that MSP activates Ras through RON, and that MSP-induced activation of Ras might be controlled by both the enhancement of catalytic exchange activity of SOS and its translocation to the membrane where its target Ras is localized.|
|B Q Li; M H Wang; H F Kung; C Ronsin; R Breathnach; E J Leonard; T Kamata|
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|Type: Journal Article|
|Title: Biochemical and biophysical research communications Volume: 216 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1995 Nov|
|Created Date: 1995-12-21 Completed Date: 1995-12-21 Revised Date: 2009-11-19|
Medline Journal Info:
|Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES|
|Languages: eng Pagination: 110-8 Citation Subset: IM|
|Biological Carcinogenesis and Development Program, SAIC/Frederick, MD 21702, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Adaptor Proteins, Signal Transducing*
Cell Membrane / metabolism
Cytosol / metabolism
GRB2 Adaptor Protein
Growth Substances / pharmacology*
Guanine Nucleotide Exchange Factors
Guanosine Diphosphate / metabolism
Guanosine Triphosphate / metabolism
Hepatocyte Growth Factor*
Phosphates / metabolism
Protein Precursors / pharmacology
Proteins / analysis, metabolism*
Receptor Protein-Tyrosine Kinases / metabolism*
Receptor, Epidermal Growth Factor / metabolism
Receptors, Cell Surface / metabolism*
Recombinant Proteins / metabolism
ras Guanine Nucleotide Exchange Factors
ras Proteins / metabolism*
|0/Adaptor Proteins, Signal Transducing; 0/Antibodies; 0/GRB2 Adaptor Protein; 0/GRB2 protein, human; 0/Grb2 protein, mouse; 0/Growth Substances; 0/Guanine Nucleotide Exchange Factors; 0/Phosphates; 0/Protein Precursors; 0/Proteins; 0/Proto-Oncogene Proteins; 0/Receptors, Cell Surface; 0/Recombinant Proteins; 0/macrophage stimulating protein; 0/ras Guanine Nucleotide Exchange Factors; 146-91-8/Guanosine Diphosphate; 67256-21-7/Hepatocyte Growth Factor; 86-01-1/Guanosine Triphosphate; EC 2.7.1.-/RON protein; EC 18.104.22.168/Receptor Protein-Tyrosine Kinases; EC 22.214.171.124/Receptor, Epidermal Growth Factor; EC 126.96.36.199/ras Proteins|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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