Document Detail


Macrophage migration inhibitory factor up-regulates matrix metalloproteinase-9 and -13 in rat osteoblasts. Relevance to intracellular signaling pathways.
MedLine Citation:
PMID:  11751895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neutral matrix metalloproteinases (MMPs) play an important role in bone matrix degradation accompanied by bone remodeling. We herein show for the first time that macrophage migration inhibitory factor (MIF) up-regulates MMP-13 (collagenase-3) mRNA of rat calvaria-derived osteoblasts. The mRNA up-regulation was seen at 3 h in response to MIF (10 microg/ml), reached the maximum level at 6-12 h, and returned to the basal level at 36 h. MMP-13 mRNA up-regulation was preceded by up-regulation of c-jun and c-fos mRNA. Tissue inhibitor of metalloproteinase (TIMP)-1 and MMP-9 (92-kDa type IV collagenase) were also up-regulated, but to a lesser extent. The MMP-13 mRNA up-regulation was significantly suppressed by genistein, herbimycin A and 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine. Similarly, a selective mitogen-activated protein kinase (MAPK) kinase (MEK)1/2 inhibitor (PD98059) and c-jun/activator protein (AP)-1 inhibitor (curcumin) suppressed MMP-13 mRNA up-regulation induced by MIF. The mRNA levels of c-jun and c-fos in response to MIF were also inhibited by PD98059. Consistent with these results, MIF stimulated phosphorylation of tyrosine, autophosphorylation of Src, activation of Ras, activation of extracellular signal-regulated kinases (ERK) 1/2, a MAPK, but not c-Jun N-terminal kinase or p38, and phosphorylation of c-Jun. Osteoblasts obtained from calvariae of newborn JunAA mice, defective in phosphorylation of c-Jun, or newborn c-Fos knockout (Fos -/- ) mice, showed much less induction of MMP-13 with the addition of MIF than osteoblasts obtained from wild-type or littermate control mice. Taken together, these results suggest that MIF increases the MMP-13 mRNA level of rat osteoblasts via the Src-related tyrosine kinase-, Ras-, ERK1/2-, and AP-1-dependent pathway.
Authors:
Shin Onodera; Jun Nishihira; Kazuya Iwabuchi; Yoshikazu Koyama; Kazuhiko Yoshida; Sakae Tanaka; Akio Minami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-12-20
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  277     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-04     Completed Date:  2002-04-15     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7865-74     Citation Subset:  IM    
Affiliation:
Department of Orthopaedics, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone and Bones / metabolism
Collagenases / biosynthesis*,  metabolism
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Fibroblasts / enzymology,  metabolism
Flavonoids / pharmacology
Genes, Dominant
MAP Kinase Kinase 1
MAP Kinase Kinase 2
Macrophage Migration-Inhibitory Factors / metabolism*
Matrix Metalloproteinase 13
Matrix Metalloproteinase 9 / biosynthesis*
Mitogen-Activated Protein Kinase Kinases / metabolism
Mitogen-Activated Protein Kinases / metabolism
Osteoblasts / enzymology*,  metabolism*
Phosphorylation
Protein Binding
Protein-Serine-Threonine Kinases / metabolism
Protein-Tyrosine Kinases / metabolism
Proto-Oncogene Proteins c-jun / metabolism
RNA, Messenger / metabolism
Rats
Recombinant Proteins / metabolism
Signal Transduction*
Time Factors
Transcription Factor AP-1 / metabolism
Tyrosine / metabolism
Up-Regulation*
p38 Mitogen-Activated Protein Kinases
src-Family Kinases / metabolism
Chemical
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Enzyme Inhibitors; 0/Flavonoids; 0/Macrophage Migration-Inhibitory Factors; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Transcription Factor AP-1; 55520-40-6/Tyrosine; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/src-Family Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 2.7.12.2/MAP Kinase Kinase 1; EC 2.7.12.2/MAP Kinase Kinase 2; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases; EC 3.4.24.-/Collagenases; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/Mmp13 protein, rat; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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