| Macrophage migration inhibitory factor in the human endometrium: expression and localization during the menstrual cycle and early pregnancy. | |
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MedLine Citation:
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PMID: 11259268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Macrophage migration inhibitory factor (MIF) was discovered as an activated T-lymphocyte-derived protein that inhibits the random migration of macrophages in vitro. Subsequently, knowledge of the physiological actions of MIF was extended to include its role as a proinflammatory cytokine that affects several functions of macrophages and lymphocytes. Previous reports have suggested an involvement of MIF in reproduction. However, no data are currently available on the presence of this cytokine in the human endometrium. In this study, the expression and tissue localization of MIF was evaluated in specimens of cycling endometrium, first trimester placenta bed biopsy, and isolated endometrial glands by Western blot analysis, immunohistochemistry, ELISA, and reverse transcription-polymerase chain reaction. The results demonstrated that MIF is expressed in human endometrium across the menstrual cycle and in early pregnancy. Immunohistochemical localization identified the protein in glandular epithelium, in stromal and predecidualized stromal cells of cycling endometrium, as well as in the decidua of first-trimester placenta. The proinflammatory features and specific actions of MIF on lymphoid cells suggest its potential involvement in several aspects of endometrial physiology. |
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Authors:
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F Arcuri; C Ricci; F Ietta; M Cintorino; S A Tripodi; I Cetin; E Garzia; F Schatz; P Klemi; R Santopietro; L Paulesu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of reproduction Volume: 64 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-03-22 Completed Date: 2001-05-21 Revised Date: 2008-08-29 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
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Languages: eng Pagination: 1200-5 Citation Subset: IM |
Affiliation:
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Institute of Pathological Anatomy and Histology and Institute of General Physiology, University of Siena, Siena 53100, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Deoxyribonucleases, Type II Site-Specific Endometrium / chemistry*, metabolism Female Gene Expression* Humans Immunohistochemistry Macrophage Migration-Inhibitory Factors / analysis*, genetics* Menstrual Cycle* Pregnancy RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Macrophage Migration-Inhibitory Factors; 0/RNA, Messenger; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific; EC 3.1.21.4/GTAC-specific type II deoxyribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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