Document Detail

Macrophage infiltration and its prognostic relevance in clear cell renal cell carcinoma.
MedLine Citation:
PMID:  21453387     Owner:  NLM     Status:  Publisher    
Most malignant tumors evidence infiltration of many macrophages. In this study, we investigated an anti-inflammatory macrophage phenotype (M2) in clear cell renal cell carcinoma (ccRCC) using CD163 and CD204 as markers. Immunostaining showed a correlation between the number of CD163(+) cells and age, sex, nuclear grade, and TNM classification. High infiltration of CD163(+) cells was significantly associated with poor clinical prognosis in univariate analysis but not in multivariate analysis. We also performed in vitro studies to examine cell-cell interactions between macrophages and cancer cells. Culture supernatants from RCC cell lines induced polarization of macrophages toward the M2 phenotype. Co-culture of macrophages with cancer cells significantly induced activation of signal transducers and activators of transcription-3 (Stat3) in the cancer cells. Direct co-culture of RCC cells with macrophages led to stronger activation of Stat3 in the cancer cells than did indirect co-culture in which transwell chamber dishes were utilized. Because RCC cells expressed membrane-type macrophage colony-stimulating factor (mM-CSF) on the cell surface, we suggested that this mM-CSF plays an important role in direct cell-cell interactions. Stat3 activation in cancer cells that was induced by co-culture with macrophages was suppressed by down-regulation of the M-CSF receptor (M-CSFR) in macrophages and by an inhibitor of M-CSFR. In conclusion, investigation of CD163(+) tumour-associated macrophages would be useful for assessment of the clinical prognosis of patients with ccRCC. Cell-cell interactions mediated by mM-CSF and M-CSFR binding may contribute to cancer cell activation.
Yoshihiro Komohara; Horlad Hasita; Koji Ohnishi; Yukio Fujiwara; Shinya Suzu; Masatoshi Eto; Motohiro Takeya
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-31
Journal Detail:
Title:  Cancer science     Volume:  -     ISSN:  1349-7006     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-4-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101168776     Medline TA:  Cancer Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Japanese Cancer Association.
Department of Cell Pathology Department of Urology, Graduate School of Medical Sciences Center for AIDS research, Kumamoto University, Kumamoto, Japan.
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