| Macrophage-colony stimulating factor as an inducer of microglial proliferation in axotomized rat facial nucleus. | |
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MedLine Citation:
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PMID: 20831658 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We analyzed the mechanism of microglial proliferation in rat axotomized facial nucleus (axotFN). In immunoblotting analysis for possible mitogens, we noticed that the amounts of macrophage-colony stimulating factor (M-CSF) increased in the axotFN for 3-7 days after transection. In contrast, the amounts of granulocyte macrophage-CSF and interleukin-3 did not significantly increase. A potential source for M-CSF was immunohistochemically verified to be microglia. Immunoblotting showed that the amounts of receptor for M-CSF (cFms) increased in the axotFN for 3-14 days after injury, and immunohistochemical staining showed that cFms is expressed in microglia. Proliferating cell nuclear antigen as a marker of proliferation was immunohistochemically identified in microglia in axotFN, and the level was found to peak 3 days after transection in immunoblotting. Hypothesizing that up-regulated M-CSF triggers the above phenomena, we investigated the effects of M-CSF on cFms and proliferating cell nuclear antigen levels in primary microglia. The biochemical experiments revealed that M-CSF induces cFms and drives the cell cycle in microglia. The neutralization of M-CSF in microglia derived from axotFN significantly reduced the proliferation. These results demonstrate that up-regulated M-CSF triggers the induction of cFms in microglia and causes the microglia to proliferate in the axotFN. |
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Authors:
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Shinichi Yamamoto; Kazuyuki Nakajima; Shinichi Kohsaka |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-12 |
Journal Detail:
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Title: Journal of neurochemistry Volume: 115 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-22 Completed Date: 2010-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 1057-67 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal of Neurochemistry © 2010 International Society for Neurochemistry. |
Affiliation:
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Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Axotomy Cell Proliferation* Cells, Cultured Facial Nerve / cytology*, metabolism* Macrophage Colony-Stimulating Factor / biosynthesis* Male Microglia / cytology*, metabolism* Rats Rats, Wistar Receptor, Macrophage Colony-Stimulating Factor / biosynthesis |
| Chemical | |
Reg. No./Substance:
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81627-83-0/Macrophage Colony-Stimulating Factor; EC 2.7.10.1/Receptor, Macrophage Colony-Stimulating Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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