Document Detail


Macrophage activation, phagocytosis and intracellular calcium oscillations induced by scorpion toxins from Tityus serrulatus.
MedLine Citation:
PMID:  19037924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The research described here is focused upon studying the activation of mice peritoneal macrophages when submitted to in vitro effects of Tityus serrulatus scorpion venom and its major toxic peptides. Several functional events were analysed, such as: cytotoxicity, spreading, extent of phagocytosis, vacuole formation and changes of internal calcium concentration. Among the main results observed, when macrophages are subjected to the effects of soluble venom of Tityus serrulatus scorpion venom, a partially purified fraction (FII) or a pure toxin (Ts1), are an increment in the percentage of phagocytosis and vacuole formation, a decrement of the spreading ability, accompanied by oscillations of internal calcium concentration. The net results demonstrate that scorpion venom or its major toxins are effective stimulators of macrophage activity; the effect of whole soluble venom or partially purified fractions is due to the toxic peptides, seen here clearly with Ts1. The possible involvement of Na+-channels in these events is discussed. A basic understanding of the underlying molecular mechanisms responsible for macrophage activation should serve as a foundation for novel drug development aimed at modulating macrophage activity.
Authors:
V L Petricevich; E Reynaud; A H Cruz; L D Possani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  154     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-28     Completed Date:  2009-04-02     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  415-23     Citation Subset:  IM    
Affiliation:
Laboratorio de Toxicología, Facultad de Medicina de la Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Signaling / drug effects*
Cell Adhesion / drug effects
Cell Survival / drug effects
Cells, Cultured
Female
Macrophage Activation / drug effects*
Macrophages, Peritoneal / drug effects,  physiology,  ultrastructure
Mice
Mice, Inbred BALB C
Phagocytosis / drug effects*
Scorpion Venoms / pharmacology*
Vacuoles / drug effects
Chemical
Reg. No./Substance:
0/Scorpion Venoms
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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