Document Detail

Macrophage and T lymphocyte apoptosis during experimental pulmonary tuberculosis: their relationship to mycobacterial virulence.
MedLine Citation:
PMID:  16421947     Owner:  NLM     Status:  MEDLINE    
The kinetics of macrophage and T lymphocyte apoptosis were determined in a well-characterized mouse model of pulmonary tuberculosis, comparing strains of intermediate (H37Rv) and high virulence (Beijing strain, code 9501000). Both strains induced a high percentage of apoptotic activated macrophages at days 1 and 3 post infection, although this was twofold lower in Beijing-infected mice. Progressive pneumonia started at day 14 (Beijing) or 21 (H37Rv) post infection. Pneumonic areas contained numerous macrophages with vacuolated cytoplasm (VM). In H37Rv infection few VM were apoptotic (8.7% at day 60), and the percentage was even lower in Beijing infection (1.4% at day 28). A high percentage of VM expressed the anti-apoptotic molecule Bcl-2 (H37Rv, 83%; Beijing, 95%). Both strains induced a progressive increase of apoptotic Th1 lymphocytes, peaking at day 60 in H37Rv infection, or 28 in Beijing infection. The peak was twofold higher in the latter. VM had strong FasL immunostaining, and confocal microscopy showed numerous apoptotic Th1 cells closely associated with them, suggesting that VM might induce apoptosis of Th1 cells. These results support the hypothesis that apoptosis of macrophages is associated with protection, while apoptosis of Th1 cells favors disease progression, and is related to the virulence of the mycobacterial strain.
Víctor Adolfo Ríos-Barrera; Victoria Campos-Peña; Diana Aguilar-León; Ledesma Ricardo Lascurain; Marco Antonio Meraz-Ríos; José Moreno; Víctor Figueroa-Granados; Rogelio Hernández-Pando
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  36     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-07     Completed Date:  2006-03-24     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  345-53     Citation Subset:  IM    
Experimental Pathology Section, Department of Pathology, Instituto Nacional de Ciencias Médicas y Nutrición, Salvador Zubirán, Tlalpan, Mexico City CP-14000, Mexico.
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MeSH Terms
Apoptosis / immunology*
Cytoplasm / immunology,  microbiology,  pathology
Disease Models, Animal
Fas Ligand Protein
Macrophages, Alveolar / immunology*,  microbiology,  pathology
Membrane Glycoproteins / immunology
Mice, Inbred BALB C
Microscopy, Confocal
Mycobacterium tuberculosis / immunology*,  pathogenicity
Pneumonia / immunology,  microbiology,  pathology
Proto-Oncogene Proteins / immunology
Species Specificity
Th1 Cells / immunology*,  microbiology,  pathology
Th2 Cells / immunology*,  microbiology,  pathology
Tuberculosis, Pulmonary / immunology*,  microbiology,  pathology
Tumor Necrosis Factors / immunology
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Fas Ligand Protein; 0/Fasl protein, mouse; 0/Membrane Glycoproteins; 0/Proto-Oncogene Proteins; 0/Tumor Necrosis Factors; 114100-40-2/Bcl2 protein, mouse

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