Document Detail

Macrophage subtypes in symptomatic carotid artery and femoral artery plaques.
MedLine Citation:
PMID:  22975154     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To compare differences in macrophage heterogeneity and morphological composition between atherosclerotic plaques obtained from recently symptomatic patients with carotid artery disease and femoral plaques from patients with severe limb ischemia.
DESIGN: Experimental study.
METHODS: Plaques were obtained from 32 patients undergoing carotid endarterectomy and 25 patients undergoing common femoral endarterectomy or lower limb bypass. Macrophages and T cell numbers were detected in plaque sections by immunohistochemistry and anti CD68 and CD3 antibodies. Dual staining for CD68 and M1- and M2-macrophage markers and morphometric analysis of hematoxylin and eosin stained plaque sections was performed.
RESULTS: Carotid plaques had significantly increased percentage areas of confluent lipid and leukocytic infiltrates. In contrast, areas of fibroconnective tissue were significantly greater in femoral plaques and percentage areas of confluent calcification and collagen were elevated. Carotid artery plaques had greater numbers per plaque area of macrophages and T cells consistent with a more inflammatory phenotype. Proportions displaying M1-activation markers were significantly increased in the carotid compared to femoral plaques whereas femoral plaques displayed a greater proportion of M2-macrophages.
CONCLUSION: Plaques from patients with recently symptomatic carotid disease have a predominance of M1-macrophages and higher lipid content than femoral plaques, consistent with a more unstable plaque.
S Shaikh; J Brittenden; R Lahiri; P A J Brown; F Thies; H M Wilson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-10
Journal Detail:
Title:  European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery     Volume:  44     ISSN:  1532-2165     ISO Abbreviation:  Eur J Vasc Endovasc Surg     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2012-12-31     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9512728     Medline TA:  Eur J Vasc Endovasc Surg     Country:  England    
Other Details:
Languages:  eng     Pagination:  491-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.
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MeSH Terms
Aged, 80 and over
Antigens, CD / analysis
Antigens, CD3 / analysis
Antigens, Differentiation, Myelomonocytic / analysis
Atherosclerosis / immunology*,  pathology,  surgery
Biological Markers / analysis
Carotid Arteries / immunology*,  pathology,  surgery
Carotid Artery Diseases / immunology*,  pathology,  surgery
Constriction, Pathologic
Femoral Artery / immunology*,  pathology,  surgery
Macrophages / classification,  immunology*,  pathology
Middle Aged
Plaque, Atherosclerotic
T-Lymphocytes / immunology
Grant Support
G0500711//Medical Research Council
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD3; 0/Antigens, Differentiation, Myelomonocytic; 0/Biological Markers; 0/CD68 antigen, human

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