Document Detail


Macrophage migration inhibitory factor provides cardioprotection during ischemia/reperfusion by reducing oxidative stress.
MedLine Citation:
PMID:  20831446     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Abstract Macrophage migration inhibitory factor (MIF) is a multifunctional protein that exhibits an intrinsic thiol protein oxidoreductase activity and proinflammatory activities. In the present study to examine intracellular MIF redox function, exposure of MIF-deficient cardiac fibroblasts to oxidizing conditions resulted in a 2.3-fold increase (p < 0.001) in intracellular ROS that could be significantly reduced by adenoviral-mediated reexpression of recombinant MIF. In an animal model of myocardial injury by ischemia/reperfusion (I/R), MIF-deficient hearts exhibited higher levels of oxidative stress than did wild-type hearts, as measured by significantly higher oxidized glutathione levels (decreased GSH/GSSG ratio), increased protein oxidation, reduced aconitase activity, and increased mitochondrial injury (increased cytochrome c release). The increased myocardial oxidative stress after I/R was reflected by larger infarct size (INF) in MIF-deficient hearts versus wild-type (WT) hearts (21 ± 6% vs. 8 ± 3% INF/LV; p < 0.05). In vivo hemodynamic measurements showed that left ventricular (LV) contractile function of MIF-deficient hearts subjected to 15-min ischemia failed to recover during reperfusion compared with WT hearts (LV developed pressure and ± dP/dt; p = 0.02). These data represent the first in vivo evidence in support of a cardioprotective role of MIF in the postischemic heart by reducing oxidative stress. Antioxid. Redox Signal. 14, 1191-1202.
Authors:
Kiyokazu Koga; Agnes Kenessey; Saul R Powell; Cristina P Sison; Edmund J Miller; Kaie Ojamaa
Related Documents :
18602426 - Glutathione peroxidase 1-deficient mice are more susceptible to doxorubicin-induced car...
11834506 - Acute protection of ischemic heart by fgf-2: involvement of fgf-2 receptors and protein...
9354516 - Vascular endothelial growth factor attenuates myocardial ischemia-reperfusion injury.
12628506 - Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract.
22963506 - Does spironolactone have a dose-dependent effect on left ventricular remodeling in pati...
17081086 - The use of tp10, soluble complement receptor 1, in cardiopulmonary bypass.
Publication Detail:
Type:  Journal Article     Date:  2011-02-05
Journal Detail:
Title:  Antioxidants & redox signaling     Volume:  14     ISSN:  1557-7716     ISO Abbreviation:  Antioxid. Redox Signal.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888899     Medline TA:  Antioxid Redox Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1191-202     Citation Subset:  IM    
Affiliation:
1 Elmezzi Graduate School of Molecular Medicine, The Feinstein Institute for Medical Research, North Shore-LIJ Health System , Manhasset, New York.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Control of mature protein function by allosteric disulfide bonds.
Next Document:  Different isoforms of glutathione peroxidase cause opposing effects during the development of allerg...