Document Detail


Macrophage Migration Inhibitory Factor Isolated from a Parasite Inhibited Th2 Cytokine Production in PBMCs of Atopic Asthma Patients.
MedLine Citation:
PMID:  22149098     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background. In a previous study, we demonstrated that the human macrophage migration inhibitory factor (MIF)-like protein (As-MIF) isolated from helminths could inhibit allergic airway inflammation via the recruitment of CD4(+)CD25(+)Foxp3(+) T cells. Objective. To evaluate the clinical importance of As-MIF as an antiasthma drug, we evaluated immune responses after recombinant As-MIF (rAs-MIF) treatment in peripheral blood mononuclear cell (PBMC) cultures. Methods. PBMC was isolated from 10 patients with atopic asthma, 8 patients with nonatopic asthma, and 12 nonatopic healthy subjects, and various concentrations of rAs-MIF were transferred into the PBMC culture medium. After 3 days, we measured the levels of T helper 2 and T helper 1 cytokines via ELISA. Results. In atopic asthma, IL-4 and IL-5 production was significantly reduced in the PBMC cultures after rAs-MIF treatment. These inhibitory effects were not observed in the nonatopic asthma group. By way of contrast, IL-10 production in the PMBC cultures was significantly increased after rAs-MIF treatment in all experimental groups. Conclusion. The results of this study are similar to those previously reported in a mouse study, suggesting that As-MIF might be a candidate for the specific treatment of asthma.
Authors:
Hye-Kyung Paark; Min Kyoung Cho; Hee Young Park; Ki Uk Kim; Yun Seong Kim; Min Ki Lee; Soon Kew Park; Dong-Hee Kim; Hak Sun Yu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-7
Journal Detail:
Title:  The Journal of asthma : official journal of the Association for the Care of Asthma     Volume:  -     ISSN:  1532-4303     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106454     Medline TA:  J Asthma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Internal Medicine, School of Medicine, Pusan National University , Busan, Republic of Korea.
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