Document Detail

Macromolecular organization and genetic mapping of a rapidly evolving chromosome-specific tandem repeat family (B77) in cotton (Gossypium).
MedLine Citation:
PMID:  9869409     Owner:  NLM     Status:  MEDLINE    
Isolation and characterization of the most prominent repetitive element families in the genome of tetraploid cotton (Gossypium barbadense L; [39]) revealed a small subset of families that showed very different properties in tetraploids than in their diploid progenitors, separated by 1-2 million years. One element, B77, was characterized in detail, and compared to the well-conserved 5S and 45S rRNA genes. The 572 bp B77 repeat was found to be concentrated in several discontinuous tandem arrays confined to a single 550 kb SalI fragment in tetraploid cotton. Genetic mapping based on the absence of the pentameric 'rung' in the G. barbadense 'ladder' showed that B77 maps to a D-subgenome chromosome. In situ hybridization supports the contention that the array is confined largely to a single chromosomal site in the D-subgenome. The B77 repeat has undergone a substantial increase in copy number since formation of tetraploid cotton from its diploid relatives. RFLPs observed among tetraploid cotton species suggest that amplification and/or rearrangement of the repeat may have continued after divergence of the five tetraploid cotton species. B77 contains many short direct repeats and shares significant DNA sequence homology with a Nicotiana alata retrotransposon Tna1-2 integrase motif. The recent amplification of B77 on linkage group D04 suggests that the D-subgenome of tetraploid cotton may be subject to different evolutionary constraints than the D-genome diploid chromosomes, which exhibit few genome-specific elements. Further, the abundance of B77 in G. gossypioides supports independent evidence that it may be the closest extant relative of the D-genome ancestor of cotton.
X Zhao; Y Ji; X Ding; D M Stelly; A H Paterson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Plant molecular biology     Volume:  38     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-01-15     Completed Date:  1999-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1031-42     Citation Subset:  IM    
Department of Soil and Crop Sciences, Texas A&M University, College Station 77843-2474, USA.
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MeSH Terms
Base Sequence
Chromosome Mapping*
DNA, Plant / chemistry,  genetics
Evolution, Molecular*
Genes, Plant*
Genetic Markers
Gossypium / genetics*
Linkage (Genetics)
Molecular Sequence Data
Multigene Family*
Repetitive Sequences, Nucleic Acid
Reg. No./Substance:
0/DNA, Plant; 0/Genetic Markers

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