| Macromolecular geometries determined with field-flow fractionation and their impact on the overlap concentration. | |
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MedLine Citation:
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PMID: 18537296 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared with viscosimetry and discussed in terms of concentration and close-packing in relation to macromolecular shape and conformation. Hence, the results show that AsFlFFF and our novel methodology enable the determination of several physical properties with high relevance for the solution behavior of polydisperse macromolecules. |
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Authors:
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Cinthia Carola Rojas; Karl-Gustav Wahlund; Björn Bergenståhl; Lars Nilsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomacromolecules Volume: 9 ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-06-09 Completed Date: 2008-07-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 1684-90 Citation Subset: IM |
Affiliation:
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Division of Food Technology, Faculty of Engineering LTH, Lund University, Post Office Box 124, S-221 00 Lund, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Complex Mixtures
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chemistry Fractionation, Field Flow / methods* Hordeum / chemistry Light Molecular Conformation Particle Size Pressure Rheology Scattering, Radiation Starch / chemistry* Viscosity |
| Chemical | |
Reg. No./Substance:
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0/Complex Mixtures; 9005-25-8/Starch |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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