Document Detail

Macromolecular diffusion and release from self-assembled beta-hairpin peptide hydrogels.
MedLine Citation:
PMID:  19100615     Owner:  NLM     Status:  MEDLINE    
Self-assembling peptide hydrogels are used to directly encapsulate and controllably release model FITC-dextran macromolecules of varying size and hydrodynamic diameters. MAX1 and MAX8 are two peptide sequences with different charge states that have been designed to intramolecularly fold and self assemble into hydrogels at physiological buffer conditions (pH 7.4, 150 mM NaCl). When self-assembly is initiated in the presence of dextran or protein probes, these macromolecules are directly encapsulated in the gel. Self-diffusion studies using fluorescence recovery after photobleaching (FRAP) and bulk release studies indicate that macromolecule mobility within, and release out of, these gels can be modulated by varying the hydrogel mesh size. The average mesh size can be modulated by simply varying the concentration of a given peptide used to construct the gel or by altering the peptide sequence. In addition, results suggest that electrostatic interactions between the macromolecules and the peptide network influence mobility and release. Depending on probe size, release half-lives can be varied from 8h to over a month.
Monica C Branco; Darrin J Pochan; Norman J Wagner; Joel P Schneider
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-12-18
Journal Detail:
Title:  Biomaterials     Volume:  30     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-01-12     Completed Date:  2009-03-05     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1339-47     Citation Subset:  IM    
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA.
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MeSH Terms
Amino Acid Sequence
Biocompatible Materials / chemistry
Dextrans / chemistry
Drug Carriers / chemistry
Fluorescein-5-isothiocyanate / analogs & derivatives,  chemistry
Fluorescence Recovery After Photobleaching
Hydrogels / chemistry*
Materials Testing
Models, Molecular
Molecular Probes / chemistry
Molecular Sequence Data
Peptides / chemistry*,  genetics
Protein Folding
Protein Structure, Secondary*
Grant Support
R01 DE016386-01/DE/NIDCR NIH HHS; R01 DE016386-04/DE/NIDCR NIH HHS
Reg. No./Substance:
0/Biocompatible Materials; 0/Drug Carriers; 0/Hydrogels; 0/Molecular Probes; 0/Peptides; 0/fluorescein isothiocyanate dextran; 3326-32-7/Fluorescein-5-isothiocyanate; 9004-54-0/Dextrans

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