Document Detail

Macrolide antibiotics induce apoptosis of human peripheral lymphocytes in vitro.
MedLine Citation:
PMID:  15325428     Owner:  NLM     Status:  MEDLINE    
We previously reported that long-term administration of macrolides (MCL) reduced the number of lymphocytes in bronchoalveolar lavage fluid (BALF) of patients with chronic lower respiratory tract disease. To investigate the anti-inflammatory activity of macrolides, we evaluated their effect on apoptosis of lymphocytes isolated from human peripheral blood. Lymphocytes treated with clarithromycin, azithromycin and josamycin at a final concentration of 200 microg/ml showed positive staining for Annexin V, Fas and Fas ligand using flow cytometry with time at 12-72 h, while other antibiotics did not. Our results suggest that macrolides induce apoptosis of lymphocytes through Fas-Fas ligand pathway and could potentially reduce the number of lymphocytes in the lungs of patients with chronic lower respiratory tract disease.
Yuji Ishimatsu; Jun-Ichi Kadota; Tetsuji Iwashita; Towako Nagata; Hiroshi Ishii; Chieko Shikuwa; Hideyuki Kaida; Hiroshi Mukae; Shigeru Kohno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of antimicrobial agents     Volume:  24     ISSN:  0924-8579     ISO Abbreviation:  Int. J. Antimicrob. Agents     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-08-24     Completed Date:  2005-02-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9111860     Medline TA:  Int J Antimicrob Agents     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  247-53     Citation Subset:  IM    
Second Department of Internal Medicine, School of Medicine, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
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MeSH Terms
Annexin A5 / analysis
Anti-Bacterial Agents / pharmacology*
Antigens, CD95
Azithromycin / pharmacology
Cells, Cultured
Clarithromycin / pharmacology
Fas Ligand Protein
Flow Cytometry
Josamycin / adverse effects
Lymphocytes / cytology,  drug effects*
Macrolides / pharmacology*
Membrane Glycoproteins / analysis
Receptors, Tumor Necrosis Factor / analysis
Time Factors
Reg. No./Substance:
0/Annexin A5; 0/Anti-Bacterial Agents; 0/Antigens, CD95; 0/FAS protein, human; 0/FASLG protein, human; 0/Fas Ligand Protein; 0/Macrolides; 0/Membrane Glycoproteins; 0/Receptors, Tumor Necrosis Factor; 16846-24-5/Josamycin; 81103-11-9/Clarithromycin; 83905-01-5/Azithromycin

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