Document Detail


Macrocyst development in Dictyostelium discoideum. III. Cell-fusion inducing factor secreted by giant cells.
MedLine Citation:
PMID:  6619205     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A factor was discovered that markedly enhances the degree of fusion activity between cells of the opposite mating-type strains, MI1 and NC4, in the sexual cycle of Dictyostelium discoideum. This factor, designated cell-fusion inducing factor (CFIF), was detected initially in supernatants taken from 24 h dark-grown mixed cultures of HM1 and NC4 cells, and subsequently shown to be secreted by giant cells - the fusion products of HM1 and NC4 cells. HM1 cells, cultured in the dark at appropriate temperature, normally acquire fusion-competence specific to NC4 cells. The addition of CFIF to such dark-grown HM1 cultures results in a marked increase in their fusion-competence. In addition, when CFIF is added to light-grown HM1 cultures, in which cells normally do not acquire the ability to fuse with NC4 cells, fusion-competence is induced. The fusion-competence of NC4 cells is unaffected by CFIF, being quite high under the culture conditions used here. Experiments, using actinomycin D, daunomycin and cycloheximide showed that the secretion of CFIF from giant cells requires synthesis of RNA and protein. Possibly, cell fusion triggers production of CFIF, which is rapidly released into the external medium.
Authors:
Y Saga; K Yanagisawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cell science     Volume:  62     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1983 Jul 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  237-48     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Products / metabolism*
Cell Cycle
Cell Fusion
Culture Media
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Daunorubicin / pharmacology
Dictyostelium / cytology*,  drug effects,  metabolism
Fungal Proteins / biosynthesis
RNA, Fungal / biosynthesis
Chemical
Reg. No./Substance:
0/Biological Products; 0/Culture Media; 0/Fungal Proteins; 0/RNA, Fungal; 20830-81-3/Daunorubicin; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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