| Macroamphiphilic components of thermophilic actinomycetes: identification of lipoteichoic acid in Thermobifida fusca. | |
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MedLine Citation:
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PMID: 18931132 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cell envelopes of gram-positive bacteria contain structurally diverse membrane-anchored macroamphiphiles (lipoteichoic acids and lipoglycans) whose functions are poorly understood. Since regulation of membrane composition is an important feature of adaptation to life at higher temperatures, we have examined the nature of the macroamphiphiles present in the thermophilic actinomycetes Thermobifida fusca and Rubrobacter xylanophilus. Following hot-phenol-water extraction and purification by hydrophobic interaction chromatography, Western blotting with a monoclonal antibody against lipoteichoic acid strongly suggested the presence of a polyglycerophosphate lipoteichoic acid in T. fusca. This structure was confirmed by chemical and nuclear magnetic resonance analyses, which confirmed that the lipoteichoic acid is substituted with beta-glucosyl residues, in common with the teichoic acid of this organism. In contrast, several extraction methods failed to recover significant macroamphiphilic carbohydrate- or phosphate-containing material from R. xylanophilus, suggesting that this actinomycete most likely lacks a membrane-anchored macroamphiphile. The finding of a polyglycerophosphate lipoteichoic acid in T. fusca suggests that lipoteichoic acids may be more widely present in the cell envelopes of actinomycetes than was previously assumed. However, the apparent absence of macroamphiphiles in the cell envelope of R. xylanophilus is highly unusual and suggests that macroamphiphiles may not always be essential for cell envelope homeostasis in gram-positive bacteria. |
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Authors:
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Obaidur Rahman; Markus Pfitzenmaier; Oxana Pester; Siegfried Morath; Stephen P Cummings; Thomas Hartung; Iain C Sutcliffe |
Publication Detail:
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Type: Journal Article Date: 2008-10-17 |
Journal Detail:
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Title: Journal of bacteriology Volume: 191 ISSN: 1098-5530 ISO Abbreviation: J. Bacteriol. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-02-03 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 152-60 Citation Subset: IM |
Affiliation:
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School of Applied Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actinobacteria
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classification,
genetics,
metabolism* Cell Wall / metabolism Chromatography, Gas Chromatography, Ion Exchange Fatty Acids / isolation & purification, metabolism Gene Amplification Gram-Positive Bacteria / genetics, metabolism Lipopolysaccharides / isolation & purification, metabolism* Phylogeny RNA, Bacterial / genetics, isolation & purification RNA, Ribosomal, 16S / genetics, isolation & purification Streptococcus agalactiae / genetics, isolation & purification, metabolism Streptomycetaceae / classification, genetics, metabolism* Teichoic Acids / isolation & purification, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lipopolysaccharides; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/Teichoic Acids; 56411-57-5/lipoteichoic acid |
| Comments/Corrections | |
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