| Machine Perfusion at 20°C Reduces Preservation Damage to Livers from Non-Heart Beating Donors. | |
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MedLine Citation:
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PMID: 21315707 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We previously reported that machine perfusion (MP) performed at 20°C enhanced the preservation of steatotic rat livers. Here, we tested whether rat livers retrieved 30min after cardiac arrest (NHBDs) were better protected by MP at 20°C than with cold storage. We compared the recovery of livers from NHBDs with organs obtained from heart beating donors (HBDs) preserved by cold storage. MP technique: livers were perfused for 6hrs with UW-G modified at 20°C. Cold storage: livers were perfused in situ and preserved with UW solution at 4°C for 6hrs. Both MP and cold storage preserved livers were reperfused with Krebs-Heinselet buffer (2hrs at 37°C). AST and LDH release and mitochondrial glutamate dehydrogenase (GDH) levels were evaluated. Parameters assessed included: bile production and biliary enzymes; tissue ATP; reduced and oxidized glutathione (GSH/GSSG); protein-SH group concentration. Livers preserved by MP at 20°C showed significantly lower hepatic damage at the end of reperfusion compared with cold storage. GDH release was significantly reduced and bile production, ATP levels, GSH/GSSG and protein-SH groups were higher in livers preserved by MP at 20°C than with cold storage. The best preserved morphology and high glycogen content was obtained with livers submitted to MP at 20°C. Liver recovery using MP at 20°C was comparable to recovery with HBDs. MP at 20°C improves cell survival and gives a better-quality of preservation for livers obtained from NHBDs and may provide a new method for the successful utilization of marginal livers. |
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Authors:
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Andrea Ferrigno; Vittoria Rizzo; Eleonora Boncompagni; Alberto Bianchi; Enrico Gringeri; Daniele Neri; Plinio Richelmi; Isabel Freitas; Umberto Cillo; Mariapia Vairetti |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-9 |
Journal Detail:
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Title: Cryobiology Volume: - ISSN: 1090-2392 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Department of Internal Medicine and Therapeutics, University of Pavia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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