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MaHog1, a Hog1-type MAP kinase gene, contributes to stress tolerance and virulence of the entomopathogenic fungus Metarhizium acridum.
MedLine Citation:
PMID:  23038805     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fungal biocontrol agents have great potential in integrated pest management. However, poor efficacy and sensitivity to various adverse factors have hampered their wide application. In eukaryotic cells, Hog1 kinase plays a critical role in stress responses. In this study, MaHog1 (GenBank accession number EFY85878), encoding a member of the Hog1/Sty1/p38 MAPK family in Metarhizium acridum, was identified. Targeted gene disruption was used to analyze the role of MaHog1 in virulence and tolerance to adverse factors. Mutants with MaHog1 depletion showed increased sensitivity to high osmotic stress, high temperature and oxidative stress, and exhibited remarkable resistance to cell wall disturbing agents. These results suggest that Hog1 kinase has conserved function in regulating multistress responses among fungi, and that MaHog1 might influence cell wall biogenesis in M. acridum. Bioassays conducted with topical inoculation and intrahemocoel injection revealed that MaHog1 is required for both penetration and postpenetration development of M. acridum. MaHog1 disruption resulted in a significant reduction in virulence, likely due to the combination of a decrease in conidial germination, a reduction in appressorium formation, and a decline in growth rate in insect haemolymph, which might be caused by impairing the fungal tolerance to various stresses during infection.
Authors:
Kai Jin; Yue Ming; Yu Xian Xia
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  -     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Chongqing University.
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