Document Detail


Ma-PbFRET: Multiple Acceptors FRET Measurement Based on Partial Acceptor Photobleaching.
MedLine Citation:
PMID:  23347498     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Fluorescence resonance energy transfer (FRET) measurement based on partial acceptor photobleaching (PbFRET) is easy to implement without external references. However, the current PbFRET methods are inapplicable to the construct with multiple acceptors, which largely increase the Förster distance. Here, we proposed a linear theory for the dependence of the acceptor photobleaching probability of construct with multiple acceptors on the photobleaching degree (x) and developed a multiple acceptors PbFRET method (Ma-PbFRET) to measure the FRET efficiency of construct with multiple acceptors (n) by measuring the fluorescence intensities of both donor and acceptor channels before and after acceptor photobleaching. The Ma-PbFRET method was validated by measuring the FRET efficiency of construct with two or three acceptors under different x in living cells. Our experimental results demonstrate that the Ma-PbFRET method is capable of exactly quantifying the FRET efficiency of construct with multiple acceptors, providing a simple and powerful tool to investigate the assembly/disassembly of biomolecular complexes with larger distance in living cells.
Authors:
Huaina Yu; Jianwei Zhang; Huali Li; Tongsheng Chen
Related Documents :
23766938 - Tmarker: a free software toolkit for histopathological cell counting and staining estim...
23991098 - Geographical analysis of the distribution and spread of human rabies in china from 2005...
23477908 - Prediction of live birth in frozen-thawed single blastocyst transfer cycles by pre-free...
20230838 - Mrna diffusion explains protein gradients in drosophila early development.
8219718 - A rich complexity emerges in phototransduction.
9046538 - Zsd: a universal parameter for precision in the ultrasonic assessment of osteoporosis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-25
Journal Detail:
Title:  Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada     Volume:  -     ISSN:  1435-8115     ISO Abbreviation:  Microsc. Microanal.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712707     Medline TA:  Microsc Microanal     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-9     Citation Subset:  -    
Affiliation:
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The fiscal impact of informal caregiving to home care recipients in Canada: How the intensity of car...
Next Document:  Birth Order Progressively Affects Childhood Height.