Document Detail

MYH9-siRNA and MYH9 mutant alleles: expression in cultured cell lines and their effects upon cell structure and function.
MedLine Citation:
PMID:  18330899     Owner:  NLM     Status:  MEDLINE    
MYH9 encodes a class II nonmuscle myosin heavy chain-A (NMHC-IIA), a widely expressed 1960 amino acid polypeptide, with translated molecular weight of 220 kDa. From studies of type II myosin in invertebrates and analogy with the skeletal and smooth muscle myosin II, NMHC-IIA is considered to be involved in diverse cellular functions, including cell shape, motility and division. The current study assessed the consequences of two separate, naturally occurring MYH9 dominant mutant alleles, MYH9(R702C) and MYH9(R705H) linked to syndromic and nonsyndromic hearing loss, respectively, upon diverse NMHC-IIA related functions in two separate cultured cell lines. MYH9-siRNA-induced inhibition of NMHC-IIA in HeLa cells or HEK293 cells resulted in alterations in their shape, actin cytoskeleton and adhesion properties. However, HeLa or HEK293 cells transfected with naturally occurring MYH9 mutant alleles, MYH9(R702C) or MYH9(R705H), as well as in vitro generated deletion derivatives, MYH9(DeltaN592) or MYH9(DeltaC570), were unaffected. The effects of MYH9-siRNA-induced suppression underline the critical role of NMHC-IIA in maintenance of cell shape and adhesion. However, the results also indicate that the NMHC-IIA mutants, R702C and R705H do not inactivate or suppress the endogenous wild type NMHC-IIA within the HeLa or HEK293 cell assay system.
Yan Li; David R Friedmann; Anand N Mhatre; Anil K Lalwani
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  65     ISSN:  1097-0169     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-23     Completed Date:  2008-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  United States    
Other Details:
Languages:  eng     Pagination:  393-405     Citation Subset:  IM    
Copyright Information:
Copyright 2008 Wiley-Liss, Inc.
Laboratory of Molecular Otology, Department of Otolaryngology, New York University School of Medicine, New York, New York 10016, USA.
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MeSH Terms
Cell Adhesion*
Cell Line
Cell Shape*
Gene Silencing
Hela Cells
Molecular Motor Proteins / genetics,  metabolism*
Myosin Heavy Chains / genetics,  metabolism*
RNA, Small Interfering / metabolism
Grant Support
Reg. No./Substance:
0/MYH9 protein, human; 0/Molecular Motor Proteins; 0/Myosin Heavy Chains; 0/RNA, Small Interfering

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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