Document Detail


MYH9-siRNA and MYH9 mutant alleles: expression in cultured cell lines and their effects upon cell structure and function.
MedLine Citation:
PMID:  18330899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MYH9 encodes a class II nonmuscle myosin heavy chain-A (NMHC-IIA), a widely expressed 1960 amino acid polypeptide, with translated molecular weight of 220 kDa. From studies of type II myosin in invertebrates and analogy with the skeletal and smooth muscle myosin II, NMHC-IIA is considered to be involved in diverse cellular functions, including cell shape, motility and division. The current study assessed the consequences of two separate, naturally occurring MYH9 dominant mutant alleles, MYH9(R702C) and MYH9(R705H) linked to syndromic and nonsyndromic hearing loss, respectively, upon diverse NMHC-IIA related functions in two separate cultured cell lines. MYH9-siRNA-induced inhibition of NMHC-IIA in HeLa cells or HEK293 cells resulted in alterations in their shape, actin cytoskeleton and adhesion properties. However, HeLa or HEK293 cells transfected with naturally occurring MYH9 mutant alleles, MYH9(R702C) or MYH9(R705H), as well as in vitro generated deletion derivatives, MYH9(DeltaN592) or MYH9(DeltaC570), were unaffected. The effects of MYH9-siRNA-induced suppression underline the critical role of NMHC-IIA in maintenance of cell shape and adhesion. However, the results also indicate that the NMHC-IIA mutants, R702C and R705H do not inactivate or suppress the endogenous wild type NMHC-IIA within the HeLa or HEK293 cell assay system.
Authors:
Yan Li; David R Friedmann; Anand N Mhatre; Anil K Lalwani
Related Documents :
18823379 - Dcun1d3, a novel uvc-responsive gene that is involved in cell cycle progression and cel...
11817569 - Transcription factors nf-kappab and ap-1/c-fos in cell response to nocodazole.
4286249 - Continuous cultivation of virus in cell suspensions by use of the lysostat.
16531319 - Regulation of connexin43 oligomerization is saturable.
3035599 - Transient changes in permeability in hela and l cells during detachment from a substrate.
22441819 - Combination of hdac and topoisomerase inhibitors in small cell lung cancer.
25129619 - Collective and individual migration following the epithelial-mesenchymal transition.
6291559 - Functional impairment of intact rat liver cells due to biological aldehydes.
20837439 - Effects of 3-tert-butyl-4-hydroxyanisole and its hydroquinone and quinone metabolites o...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  65     ISSN:  1097-0169     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-23     Completed Date:  2008-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  United States    
Other Details:
Languages:  eng     Pagination:  393-405     Citation Subset:  IM    
Copyright Information:
Copyright 2008 Wiley-Liss, Inc.
Affiliation:
Laboratory of Molecular Otology, Department of Otolaryngology, New York University School of Medicine, New York, New York 10016, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Adhesion*
Cell Line
Cell Shape*
Gene Silencing
Hela Cells
Humans
Molecular Motor Proteins / genetics,  metabolism*
Mutation
Myosin Heavy Chains / genetics,  metabolism*
RNA, Small Interfering / metabolism
Transfection
Grant Support
ID/Acronym/Agency:
DC005199/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/MYH9 protein, human; 0/Molecular Motor Proteins; 0/Myosin Heavy Chains; 0/RNA, Small Interfering

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Overexpression of HmgD causes the failure of pupariation in Drosophila by affecting ecdysone recepto...
Next Document:  Treponema denticola Msp-deduced peptide conjugate, P34BSA, promotes RhoA-dependent actin stress fibe...