| MUC5B Is the predominant mucin glycoprotein in chronic otitis media fluid. | |
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MedLine Citation:
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PMID: 20531251 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chronic otitis media (COM), e.g. "glue" ear is characterized by middle ear effusion and conductive hearing loss. Although mucous glycoproteins (mucins), which contribute to increased effusion viscosity, have been analyzed in ear tissue specimens, no studies have been reported that characterize the molecular identity of secreted mucin proteins present in actual middle ear fluid. For this study, effusions from children with COM undergoing myringotomy at Children's National Medical Center, Washington, DC were collected. These were solubilized and gel fractionated, and the protein content was identified using a liquid chromatography tandem mass spectrometry (LC-MS/MS) proteomics approach. Western blot analyses with mucin specific antibodies and densitometry were performed to validate the mass spectrometry findings. LC-MS/MS results identified mucin MUC5B by >26 unique peptides in six of six middle ear effusion samples, whereas mucin MUC5AC was only identified in one of six middle ear effusions. These findings were validated by Western blot performed on the same six and on an additional 11 separate samples where densitometry revealed on average a 6.4-fold increased signal in MUC5B when compared with MUC5AC (p = 0.0009). In summary, although both MUC5AC and MUC5B mucins are detected in middle ear effusions, MUC5B seems to be predominant mucin present in COM secretions. |
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Authors:
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Diego Preciado; Samita Goyal; Michael Rahimi; Alan M Watson; Kristy J Brown; Yetrib Hathout; Mary C Rose |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pediatric research Volume: 68 ISSN: 1530-0447 ISO Abbreviation: Pediatr. Res. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-13 Completed Date: 2010-11-30 Revised Date: 2011-06-20 |
Medline Journal Info:
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Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
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Languages: eng Pagination: 231-6 Citation Subset: IM |
Affiliation:
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Department of Pediatric Otolaryngology-Head and Neck Surgery, Center for Genetic Medicine Research, Children's National Medical Center, 111 Michigan Avenue Northwest, Washington, DC 20001, USA. dpreciad@cnmc.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Child Chromatography, Liquid District of Columbia Electrophoresis, Polyacrylamide Gel Exudates and Transudates / chemistry* Humans Mucin-5B / analysis* Otitis Media with Effusion / metabolism* Tandem Mass Spectrometry |
| Grant Support | |
ID/Acronym/Agency:
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1P30HD40677/HD/NICHD NIH HHS; 5R24HD050846/HD/NICHD NIH HHS; HL090020-01/HL/NHLBI NIH HHS; K12 HL090020-01/HL/NHLBI NIH HHS; K12 HL090020-05/HL/NHLBI NIH HHS; R24 HD050846-01/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/MUC5B protein, human; 0/Mucin-5B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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