Document Detail


MTHFR C677T and factor V Leiden in recurrent pregnancy loss: a study among an endogamous group in North India.
MedLine Citation:
PMID:  19839754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: In our study an attempt has been made to find the prevalence of genetic thrombophilia in recurrent pregnancy loss (RPL). METHODS: Methylene tetrahydrofolate reductase C677 (MTHFR C677T) and factor V Leiden (FVL) were investigated in 84 Rajput women with two or more pregnancy losses and in 80 age- and ethnicity-matched healthy controls. Restriction digestions of polymerase chain reaction product with HinfI and Mnl I were used for MTHFR C677T and FVL polymorphism detection, respectively. RESULTS: MTHFR C677T mutation was found in 9/84 patients (10.71%) and 2/80 controls (2.5%), yielding an odds ratio (OR) for RPL related to MTHFR C677T of 4.68 (95% confidence interval [CI] = 0.98-22.37; p = 0.03). FVL was found in 4/84 patients (4.76%) and none among the controls, yielding a modified OR for RPL related to FVL of 9.00 (95% CI = 0.48-169.9; p = 0.05). Both, MTHFR C677T and FVL were not found to be significantly more prevalent in patients than controls as a whole. However, MTHFR C677T showed significant association with early pregnancy loss (OR = 6.3; 95% CI = 1.22-32.85; p-value = 0.03; Bonferroni-corrected p-value = 0.04). CONCLUSIONS: Our study supports the association between MTHFR C677T and patients with early RPL among north Indian Rajputs and strengthens the notion that thrombophilia plays a role in this clinical entity.
Authors:
Rupak Mukhopadhyay; Kallur N Saraswathy; Pradeep K Ghosh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetic testing and molecular biomarkers     Volume:  13     ISSN:  1945-0257     ISO Abbreviation:  Genet Test Mol Biomarkers     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-02-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101494210     Medline TA:  Genet Test Mol Biomarkers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  861-5     Citation Subset:  IM    
Affiliation:
Department of Anthropology, University of Delhi, Delhi, India. rupak_du@rediffmail.com
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MeSH Terms
Descriptor/Qualifier:
Abortion, Spontaneous / etiology*
Adult
Factor V / genetics*
Female
Genetic Predisposition to Disease*
Humans
India
Methylenetetrahydrofolate Reductase (NADPH2) / genetics*
Pregnancy
Thrombophilia / complications,  genetics*
Young Adult
Chemical
Reg. No./Substance:
0/factor V Leiden; 9001-24-5/Factor V; EC 1.5.1.20/Methylenetetrahydrofolate Reductase (NADPH2)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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