| MRI of magnetically labeled mesenchymal stem cells in hepatic failure model. | |
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MedLine Citation:
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PMID: 21105195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To track intravascularly transplanted mesenchymal stem cells (MSCs) labeled with superparamagnetic iron oxide (SPIO) by using magnetic resonance imaging (MRI) in an experimental rabbit model of hepatic failure. METHODS: Human MSCs labeled with FDA-approved SPIO particles (Feridex) were transplanted via the mesenteric vein into rabbits (n = 16) with carbon tetrachloride-induced hepatic failure. Magnetic resonance (MR) examinations were performed with a 3.0 T clinical scanner immediately before and 2 h and 1, 3, and 7 d after transplantation. Signal intensity (SI) changes on T2*-weighted MRI were measured, and correlation between MR findings and histomorphologic findings was also investigated. RESULTS: SI on T2*-weighted MRI decreased significantly in the liver 2 h after injection of human MSCs and returned gradually to the levels found before injection in 7 d. Changes in SI in the liver at 2 h, 1, 3, and 7 d were 41.87% ± 9.63%, 10.42% ± 4.3%, 5.12% ± 1.9%, 3.75% ± 1.2%, respectively (P < 0.001). Histologic analyses confirmed the presence of MSCs in the liver, localized mainly in the sinusoids in early period (2 h and 1 d) and concentrated to the border zone in late period (3 and 7 d). The number of iron-positive cells in the liver at 2 h and on 1, 3 and 7 d after transplantation was 29.2 ± 4.8, 10.1 ± 3.7, 6.7 ± 2.2, and 5.8 ± 2.1, respectively (P = 0.013). CONCLUSION: Intravascularly injected SPIO-labeled MSCs in an experimental rabbit model of hepatic failure can be detected and followed with MRI. |
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Authors:
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Kyu Ri Son; Se Young Chung; Hyo-Cheol Kim; Hoe Suk Kim; Seung Hong Choi; Jeong Min Lee; Woo Kyung Moon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: World journal of gastroenterology : WJG Volume: 16 ISSN: 1007-9327 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-03-04 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China |
Other Details:
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Languages: eng Pagination: 5611-5 Citation Subset: IM |
Affiliation:
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Department of Radiology, Seoul National University College of Medicine, Institute of Radiation Medicine, Seoul National University Medical Research Center, and Chongno-gu, Seoul 110-744, South Korea. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Tetrachloride Cell Movement Cells, Cultured Contrast Media / diagnostic use* Dextrans / diagnostic use* Disease Models, Animal Feasibility Studies Humans Injections, Intravenous Liver / pathology* Liver Failure / chemically induced, pathology, surgery* Magnetic Resonance Imaging* Magnetite Nanoparticles / diagnostic use* Mesenchymal Stem Cell Transplantation* Mesenteric Veins Rabbits Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Magnetite Nanoparticles; 56-23-5/Carbon Tetrachloride; 9004-54-0/Dextrans; G6N3J05W84/ferumoxides |
| Comments/Corrections | |
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