Document Detail

MRI of magnetically labeled mesenchymal stem cells in hepatic failure model.
MedLine Citation:
PMID:  21105195     Owner:  NLM     Status:  MEDLINE    
AIM: To track intravascularly transplanted mesenchymal stem cells (MSCs) labeled with superparamagnetic iron oxide (SPIO) by using magnetic resonance imaging (MRI) in an experimental rabbit model of hepatic failure.
METHODS: Human MSCs labeled with FDA-approved SPIO particles (Feridex) were transplanted via the mesenteric vein into rabbits (n = 16) with carbon tetrachloride-induced hepatic failure. Magnetic resonance (MR) examinations were performed with a 3.0 T clinical scanner immediately before and 2 h and 1, 3, and 7 d after transplantation. Signal intensity (SI) changes on T2*-weighted MRI were measured, and correlation between MR findings and histomorphologic findings was also investigated.
RESULTS: SI on T2*-weighted MRI decreased significantly in the liver 2 h after injection of human MSCs and returned gradually to the levels found before injection in 7 d. Changes in SI in the liver at 2 h, 1, 3, and 7 d were 41.87% ± 9.63%, 10.42% ± 4.3%, 5.12% ± 1.9%, 3.75% ± 1.2%, respectively (P < 0.001). Histologic analyses confirmed the presence of MSCs in the liver, localized mainly in the sinusoids in early period (2 h and 1 d) and concentrated to the border zone in late period (3 and 7 d). The number of iron-positive cells in the liver at 2 h and on 1, 3 and 7 d after transplantation was 29.2 ± 4.8, 10.1 ± 3.7, 6.7 ± 2.2, and 5.8 ± 2.1, respectively (P = 0.013).
CONCLUSION: Intravascularly injected SPIO-labeled MSCs in an experimental rabbit model of hepatic failure can be detected and followed with MRI.
Kyu Ri Son; Se Young Chung; Hyo-Cheol Kim; Hoe Suk Kim; Seung Hong Choi; Jeong Min Lee; Woo Kyung Moon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-24     Completed Date:  2011-03-04     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  5611-5     Citation Subset:  IM    
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MeSH Terms
Carbon Tetrachloride
Cell Movement
Cells, Cultured
Contrast Media / diagnostic use*
Dextrans / diagnostic use*
Disease Models, Animal
Feasibility Studies
Injections, Intravenous
Liver / pathology*
Liver Failure / chemically induced,  pathology,  surgery*
Magnetic Resonance Imaging*
Magnetite Nanoparticles / diagnostic use*
Mesenchymal Stem Cell Transplantation*
Mesenteric Veins
Time Factors
Reg. No./Substance:
0/Contrast Media; 0/Magnetite Nanoparticles; CL2T97X0V0/Carbon Tetrachloride; G6N3J05W84/ferumoxides; K3R6ZDH4DU/Dextrans

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