| MRI of macrophages in infectious knee synovitis. | |
| | |
MedLine Citation:
|
PMID: 20489072 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: The objective of our study was to prospectively evaluate macrophage imaging using ultrasmall superparamagnetic iron oxide (USPIO)-enhanced MRI to depict bacterial knee infection in an experimental rabbit model. SUBJECTS AND METHODS: Unilateral knee infection was induced by intraarticular injection of Staphylococcus aureus in eight rabbits. The contralateral knees were used as internal controls. After a mean interval of 3 days, two MRI sessions (3-T MRI) were performed before and 24 hours after IV administration of USPIO. The protocol included T1-weighted spin-echo, T2-weighted spin-echo, and T2*-weighted gradient-echo images. A gadolinium-enhanced T1-weighted sequence was also performed in the first session to optimize detection of synovial hyperplasia. MR data were analyzed qualitatively and quantitatively and compared with histopathologic findings (H and E stain and Perls Prussian blue stain). Signal-to-noise ratio changes after USPIO administration were compared using Wilcoxon's signed rank test. RESULTS: All inoculated knees presented infectious synovitis with intense infiltration of iron-loaded macrophages. In these infected knees, signal loss was determined visually and quantitatively on T1-weighted (p < 0.01), T2-weighted (p < 0.01), and T2*-weighted images (p < 0.01) 24 hours after USPIO administration, reflecting the presence of USPIO-loaded macrophages in the synovium. In contrast, no significant MR signal changes were observed in the control knees (p = 0.07-0.48), which presented a normal synovium without infiltration of iron-loaded macrophages. CONCLUSION: Macrophage imaging using USPIO-enhanced MRI can depict infectious knee synovitis. |
| | |
Authors:
|
Guillaume Bierry; Fran?ois Jehl; Agn?s Neuville; Sophie Lefevre; Philippe Robert; St?phane Kremer; Jean-Louis Dietemann |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: AJR. American journal of roentgenology Volume: 194 ISSN: 1546-3141 ISO Abbreviation: AJR Am J Roentgenol Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-21 Completed Date: 2010-06-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7708173 Medline TA: AJR Am J Roentgenol Country: United States |
Other Details:
|
Languages: eng Pagination: W521-6 Citation Subset: AIM; IM |
Affiliation:
|
Department of Radiology, University Hospital of Strasbourg, 67098 Strasbourg, France. guillaume.bierry@chru-strasbourg.fr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Contrast Media Dextrans Disease Models, Animal Ferrosoferric Oxide Image Processing, Computer-Assisted Knee Joint / pathology Macrophages / pathology* Magnetic Resonance Imaging / methods* Meglumine Organometallic Compounds Prospective Studies Rabbits Statistics, Nonparametric Synovitis / microbiology, pathology* |
| Chemical | |
Reg. No./Substance:
|
0/Contrast Media; 0/Organometallic Compounds; 0/ferumoxtran-10; 0/gadoterate meglumine; 1317-61-9/Ferrosoferric Oxide; 6284-40-8/Meglumine; 9004-54-0/Dextrans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Elbow Stiffness: Effectiveness of Conventional Radiography and CT to Explain Osseous Causes.
Next Document: Multiplanar Sinus CT: A Systematic Approach to Imaging Before Functional Endoscopic Sinus Surgery.