Document Detail


MR1B, a natural spliced isoform of the MHC-related 1 protein, is expressed as homodimers at the cell surface and activates MAIT cells.
MedLine Citation:
PMID:  23457030     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The MHC-related 1 (MR1) protein is a monomorphic, evolutionarily conserved MHC class I-like molecule, which is necessary for the development and functions of mucosal-associated invariant T (MAIT) cells, a new subset of innate-like lymphocytes. Multiple isoforms of the MR1 gene are naturally transcribed, but only the full-length MR1A has been analyzed so far. Using transfected cell lines expressing an alternative spliced transcript, MR1B, characterized by the absence of the α3 extracellular domain, we show that MR1B is transcribed and glycosylated but remains in an immature (endoglycosidase H-sensitive) state. MR1B mostly accumulates in the ER, without interacting with proteins of the peptide-loading complex such as tapasin. Interestingly, it is nevertheless found expressed at the cell surface, independently of β2-microglobulin, in a homodimeric form. MR1B is functional as its overexpression induces MAIT cell activation in vitro in the presence of bacteria. Altogether, these data show that MR1B displays several remarkable features, and probably plays a physiological role complementary to MR1A with respect to MAIT cell development and/or function.
Authors:
Julien Lion; Veronique Debuysscher; Anais Wlodarczyk; Ahmed Hodroge; Nacer-Eddine Serriari; Ludivine Choteau; Hakim Ouled-haddou; Maggy Plistat; Kaiss Lassoued; Olivier Lantz; Emmanuel Treiner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  43     ISSN:  1521-4141     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-25     Completed Date:  2013-06-17     Revised Date:  2013-10-29    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1363-73     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Avenir Group Inserm UMR925, Amiens, France.
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing*
Cell Line
Cell Membrane / genetics,  immunology*
Dimerization
Gene Expression
Histocompatibility Antigens Class I / genetics*,  immunology
Humans
Leukocytes, Mononuclear / cytology,  immunology,  metabolism*
Lymphocyte Activation
Mucous Membrane / cytology,  immunology
Plasmids
Primary Cell Culture
Protein Isoforms / genetics,  immunology
T-Lymphocytes / cytology,  immunology,  metabolism*
Transfection
Chemical
Reg. No./Substance:
0/Histocompatibility Antigens Class I; 0/MR1 protein, human; 0/Protein Isoforms

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