| MR imaging of inflammation during myelin-specific T cell-mediated autoimmune attack in the EAE mouse spinal cord. | |
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MedLine Citation:
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PMID: 19949987 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: The purpose of this study is to detect myelin-specific T cells, key pathological mediators in early multiple sclerosis, and the corresponding animal model, experimental autoimmune encephalomyelitis (EAE), in the mouse spinal cord. PROCEDURES: T cells were labeled with the iron-based, magnetic resonance (MR) contrast reagent, Feridex, and the transfection reagent, protamine sulfate, resulting in approximately 100% iron-labeling efficiency. Feridex-labeling did not alter the induction of EAE by T cells, and recipients were imaged by a 12-T MR instrument. RESULTS: Focal hypointense lesions were resolvable to gray or white matter of the lumbar spinal cord in T(2)-weighted images of the recipients of Feridex-labeled T cells. Lesions corresponded to histological evidence of inflammatory lesions and iron-labeled cells in eight-of-eight mice. In contrast, hypointense lesions were not observed eight-of-eight recipients of unlabeled T cells. CONCLUSIONS: These results demonstrate and provide methodologies for labeling, detecting, and extracting MRI-detectable foci of iron-labeled cells. |
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Authors:
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Kristine M Robinson; Jeffrey M Njus; Daniel A Phillips; Thomas M Proctor; William D Rooney; Richard E Jones |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-12-01 |
Journal Detail:
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Title: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging Volume: 12 ISSN: 1860-2002 ISO Abbreviation: Mol Imaging Biol Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-06 Completed Date: 2010-09-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101125610 Medline TA: Mol Imaging Biol Country: United States |
Other Details:
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Languages: eng Pagination: 240-9 Citation Subset: IM |
Affiliation:
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V.A. Medical Center, VA R&D 23, Bldg 101, 3710 SW US Veteran's Hospital Rd, Portland, OR 97239, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoimmunity / immunology* Encephalomyelitis, Autoimmune, Experimental / immunology*, pathology Inflammation / diagnosis, immunology* Iron / metabolism Magnetic Resonance Imaging* Mice Myelin Sheath / immunology*, pathology Protamines / metabolism Spinal Cord / immunology*, pathology Staining and Labeling T-Lymphocytes / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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R01-NS040801/NS/NINDS NIH HHS; R01NS039122/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Protamines; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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