| MPTP-induced reactive oxygen species promote cell death through a gradual activation of caspase-3 without expression of GRP78/Bip as a preventive measure against ER stress in PC12 cells. | |
| | |
MedLine Citation:
|
PMID: 12770613 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Glucose-regulated protein 78 (GRP78)/Immunoglobulin binding protein (Bip) is a chaperone which functions to protect cells from endoplasmic reticulum (ER) stress. GRP78/Bip is expressed following ER stress induced by thapsigargin, tunicamycin or chemical factors. However, the mechanism of progression of ER stress against stress factors is still obscure. We examined whether reactive oxygen species (ROS) were involved in GRP78/Bip expression and caspase-3 activity was induced in PC12 cells using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to produce ROS. We report that PC12 cells lost viability in the presence of MPTP for 24 hours as a partial effect of ROS. We also show that N-acetyl-L-cysteine diminished the MPTP-induced apoptosis with expunction of ROS. Furthermore, we observed that GRP78/Bip was not up-regulated and the caspase-3 activity was increased in the presence of MPTP. These results suggest that insubstantial ROS do not contribute to the ER stress-mediated cell death while caspase-3 is involved in ROS-promoted cell death in MPTP-treated cells. |
| | |
Authors:
|
Koji Shimoke; Motoshige Kudo; Toshihiko Ikeuchi |
Related Documents
:
|
3236003 - Resonance raman spectroscopy of hemoglobin in intact cells: a probe of oxygen uptake by... 17543233 - The other side of the coin: beneficiary effect of 'oxidative burst' upsurge with t11ts ... 9242553 - Human herpesvirus 6 (hhv-6)-positive burkitt's lymphoma: establishment of a novel cell ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Life sciences Volume: 73 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2003 Jun |
Date Detail:
|
Created Date: 2003-05-28 Completed Date: 2003-06-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
|
Languages: eng Pagination: 581-93 Citation Subset: IM |
Affiliation:
|
Laboratory of Neurobiology, Faculty of Engineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan. shimoke@ipcku.kansai-u.ac/jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
/
pharmacology* Animals Carrier Proteins / biosynthesis* Caspase 3 Caspases / biosynthesis* Cell Survival / drug effects Endoplasmic Reticulum / enzymology, metabolism* Enzyme Activation / drug effects Heat-Shock Proteins* Lipid Peroxidation / drug effects Lipid Peroxides / analysis Molecular Chaperones / biosynthesis* Oxidative Stress / drug effects* PC12 Cells Rats Reactive Oxygen Species / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Carrier Proteins; 0/Heat-Shock Proteins; 0/Lipid Peroxides; 0/Molecular Chaperones; 0/Reactive Oxygen Species; 0/molecular chaperone GRP78; 28289-54-5/1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Synergistic effect of angiopoietin-1 and vascular endothelial growth factor on neoangiogenesis in hy...
Next Document: The regulation of inducible nitric oxide synthase gene expression induced by lipopolysaccharide and ...