| MPF2-Like MADS-Box Genes Affecting Expression of SOC1 and MAF1 are Recruited to Control Flowering Time. | |
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MedLine Citation:
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PMID: 22539207 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A complex and intricate network of genes responding to various developmental and environmental signals control floral transition in plants. MADS-box genes are the key regulators and major contributors with regard to flowering time determination. Previously, MPF2-like genes belonging to the STMADS11 superclade were duplicated into MPF2-like-A and MPF2-like-B in Withania (WSA206 and WSB206) and Tubocapsicum (TAB 201). The present study was conducted to determine the effect of MPF2-like genes on flowering time by analyzing 35S:MPF2-like transgenic Arabidopsis plants as well as to probe their effects on the expression of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1, a floral promoter) and MADS AFFECTING FLOWERING 1 (MAF1, a floral repressor) genes. The overexpression of WSA206 (35S:MPF2-like-A) moderately promoted flowering, while that of WSB206 and TAB 201 (35S:MPF2-like-B) exhibited no effects on floral transition. Concomitantly, an elevation in SOC1 transcript abundance and a reduction for MAF1 transcript levels were observed in 35S:WSA206 transgenic plants. Nucleotide diversity analysis indicated an extraordinary 8 aa extension at the C-terminus of the WSA206 protein. Ectopic expression of a truncated WSA206-version without these 8 aa (WSA206ΔC246) and of MPF2-like-B-versions elongated by these 8 aa (WSB206∇C257 and TAB 201∇C257) in Arabidopsis revealed an ambiguous role of the 8 aa signature in floral transition. It may influence a protein's ability to modulate flowering time but is neither sufficient nor strictly necessary for early flowering. Nevertheless, the 8 aa extension influences the expression of SOC1 and MAF1 in MPF2-like derivative constructs. Our studies provide insight into the role of MPF2-like genes in phase transition by interacting with SOC1 and MAF1 genes, thereby also pointing to their significance as potential candidates for modifying flowering in crop plants in the future. |
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Authors:
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Muhammad Ramzan Khan; Irfan Ullah Khan; Ghulam Muhammad Ali |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-28 |
Journal Detail:
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Title: Molecular biotechnology Volume: - ISSN: 1559-0305 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Center, Park Road, Islamabad, Pakistan, rkhan_75@yahoo.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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