| MOPC 104E plasmacytoma functional heterogeneity and maturational potential in culture. | |
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MedLine Citation:
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PMID: 6825114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MOPC 104E plasmacytoma secretes an immunoglobulin M (IgM) paraprotein which reacts specifically with dextran B-1355. We separated plasmacytoma cells into subfractions by density gradient centrifugation. The majority of cells isolated from the peritoneal washes 3 days following i.p. transplantation of tumor were in active cell cycle and had a density of 1.055 or 1.065 g/ml. Cells isolated from 9-day-old ascites were confined to the heavy-density fraction (1.085 g/ml). The majority of these cells were not in proliferative phase. Functional analysis of the fractions using rosette formation and plaque formation assays indicated that cells in active cell cycle had less surface IgM and secreted less IgM than did cells not in cell cycle. An attempt was made to establish a cultured cell line of MOPC 104E plasmacytoma. The majority of the cells of the continuously cultured cell line were in active cell cycle, had less surface IgM, and secreted less IgM. Cultured cells acquired more cell surface IgM and actively secreted IgM following their secondary colonization in 0.8% methylcellulose. These studies showed that the new line of MOPC 104E retained the properties of the original ascites tumor. An important feature of these studies is that the density of cells within a colony is a stable property and is probably not related to cell cycle. |
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Authors:
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T Miura; R N Hiramoto; V K Ghanta |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 43 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-04-07 Completed Date: 1983-04-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 953-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle Cell Separation Cells, Cultured Centrifugation, Density Gradient DNA Replication DNA, Neoplasm / biosynthesis Female Immunoglobulin M / secretion Kinetics Mice Mice, Inbred BALB C Plasmacytoma / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AG 02327/AG/NIA NIH HHS; CA 25965/CA/NCI NIH HHS; CA 27765/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Neoplasm; 0/Immunoglobulin M |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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