Document Detail


Matrix metalloproteinase-7 coordinates airway epithelial injury response and differentiation of ciliated cells.
MedLine Citation:
PMID:  23258229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Matrix metalloproteinase-7 (MMP7) expression is quickly up-regulated after injury, and functions to regulate wound repair and various mucosal immune processes. We evaluated the global transcriptional response of airway epithelial cells from wild-type and Mmp7-null mice cultured at an air-liquid interface. The analysis of differentially expressed genes between genotypes after injury revealed an enrichment of functional categories associated with inflammation, cilia, and differentiation. Because these analyses suggested that MMP7 regulated ciliated cell formation, we evaluated the recovery of the airway epithelium in wild-type and Mmp7-null mice in vivo after naphthalene injury, which revealed augmented ciliated cell formation in the absence of MMP7. Moreover, in vitro studies evaluating cell differentiation in air-liquid interface cultures also showed faster ciliated cell production under Mmp7-null conditions compared with wild-type conditions. These studies identified a new role for MMP7 in attenuating ciliated cell differentiation during wound repair.
Authors:
Sina A Gharib; William A Altemeier; Laura S Van Winkle; Charles G Plopper; Saundra Y Schlesinger; Catherine A Buell; Rena Brauer; Vivian Lee; William C Parks; Peter Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-12-20
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  48     ISSN:  1535-4989     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-04     Completed Date:  2013-07-15     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  390-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / physiology
Cells, Cultured
Epithelial Cells / enzymology,  pathology*
Gene Expression
Genotype
Lung Injury / enzymology,  genetics,  physiopathology
Male
Matrix Metalloproteinase 7 / genetics,  metabolism*
Mice
Mice, Inbred C57BL
Respiratory Mucosa / enzymology,  injuries*,  innervation*,  pathology,  physiopathology*
Transcription, Genetic
Transcriptome
Up-Regulation
Wound Healing / genetics*,  physiology
Grant Support
ID/Acronym/Agency:
HL029594/HL/NHLBI NIH HHS; HL072730/HL/NHLBI NIH HHS; HL084396/HL/NHLBI NIH HHS; HL086883/HL/NHLBI NIH HHS; HL103868/HL/NHLBI NIH HHS; K08 HL084396/HL/NHLBI NIH HHS; R01 HL103868/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.24.23/MMP7 protein, human; EC 3.4.24.23/Matrix Metalloproteinase 7
Comments/Corrections

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