Document Detail


MMP13 promoter polymorphism is associated with atherosclerosis in the abdominal aorta of young black males.
MedLine Citation:
PMID:  12392760     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies suggested that remodeling of connective tissue is important in progression of atherosclerosis. We investigated the importance of matrix metalloproteinase 13 (MMP13), in the pathogenesis of atherosclerosis using 995 samples from the Pathobiological Determinants of Atherosclerosis in Youth collection in an association study. We identified two new MMP13 promoter polymorphisms. The genotype for one of the MMP13 polymorphisms was associated with fibrous plaque (P=0.024) in black males. Immunohistochemistry using antibodies for MMP13 showed that MMP13 is expressed in all layers of the aorta. In-vitro transfection experiments with reporter gene constructs and electrophoretic mobility-shift assays showed that the MMP13 polymorphism was a functional variant. MMP13 is therefore, a genetic risk factor for extent of fibrous plaque in the abdominal aorta in young black males. Elucidation of the currently unknown mechanism of the MMP13 polymorphism's action may provide for pharmacological intervention to reduce the severity of atherosclerotic changes in susceptible individuals.
Authors:
Sungpil Yoon; Helena Kuivaniemi; Zoran Gatalica; Jane M Olson; Giovanna Butticè; Siqin Ye; Brent A Norris; Gray T Malcom; Jack P Strong; Gerard Tromp
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Matrix biology : journal of the International Society for Matrix Biology     Volume:  21     ISSN:  0945-053X     ISO Abbreviation:  Matrix Biol.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-23     Completed Date:  2003-04-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9432592     Medline TA:  Matrix Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  487-98     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Elsevier Science B.V. and International Society of Matrix Biology
Affiliation:
The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detriot, MI 48201, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
African Continental Ancestry Group / genetics*
Aorta, Abdominal* / metabolism
Arteriosclerosis / genetics*,  metabolism
Collagenases / genetics*,  metabolism
Gene Frequency
Humans
Immunohistochemistry
Male
Matrix Metalloproteinase 13
Polymorphism, Genetic*
Promoter Regions, Genetic / genetics*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
HG01577/HG/NHGRI NIH HHS; RR03655/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.24.-/Collagenases; EC 3.4.24.-/MMP13 protein, human; EC 3.4.24.-/Matrix Metalloproteinase 13

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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