Document Detail

The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography.
MedLine Citation:
PMID:  16830066     Owner:  NLM     Status:  MEDLINE    
Matrix metalloproteinases (MMPs) have been expressed during root development and periodontal tissue formation, whereas it is not known if these MMP molecules are enzymatically active to degrade the extracellular matrices (ECMs). The present study was designed to investigate the gelatinolytic and collagenolytic activity in rat molar root and incisor development. Three-week old rat mandibles were frozen and cut without fixation or decalcification and processed for in situ zymography using substrates gelatin and collagen. The enzymatic activity was assessed according to the intensity of fluorescence due to the lysis of the substrates. Odontoblasts, predentin, cementum, bone and the enamel matrix showed the high activity. The present study demonstrated MMP activity in calcified tissues using in situ zymography for the first time and the possible involvement of the MMP activity in molar root and incisor development and periodontal tissue formation.
Ichiro Sakuraba; Junko Hatakeyama; Yuji Hatakeyama; Ichiro Takahashi; Hideaki Mayanagi; Yasuyuki Sasano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-08
Journal Detail:
Title:  Journal of molecular histology     Volume:  37     ISSN:  1567-2387     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2009-04-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  87-93     Citation Subset:  IM    
Division of Pediatric Dentistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.
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MeSH Terms
Dental Cementum / enzymology
Dental Enamel / enzymology
Dentin / enzymology
Extracellular Matrix / enzymology
Frozen Sections
Matrix Metalloproteinases / metabolism*
Odontoblasts / cytology,  enzymology
Rats, Wistar
Tooth Root / enzymology*,  growth & development
Reg. No./Substance:
EC 3.4.24.-/Matrix Metalloproteinases

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