Document Detail

MMP-9 as a marker of inflammation in tracheal epithelial lining fluid (TELF) and in bronchoalveolar fluid (BALF) of COPD horses.
MedLine Citation:
PMID:  11266061     Owner:  NLM     Status:  MEDLINE    
Gelatinolytic activity was analysed to study whether elevated activity previously found at the tracheal level of the respiratory tract of horses with chronic obstructive pulmonary disease (COPD) could also be found at the lower part of the respiratory tract. Furthermore, presence and significance of the gelatinolytic matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in respiratory secretions of healthy and COPD horses were determined. Elevated gelatinolytic matrix metalloproteinases were detected in bronchoalveolar and tracheobronchial secretions from COPD horses. The main pathologically elevated MMP was characterised to be MMP-9. Significantly increased MMP-9 activities as measured by gelatin zymography and Western blotting were found in all the respiratory samples from COPD horses compared to healthy horses. Elevation of active MMP-9 paralleled with increased gelatinase-associated lipocalin levels. Bronchoalveolar lavage fluid (BALF) epithelial cells, macrophages, neutrophils and lymphocytes expressed MMP-9 immunoreactivity demonstrated by immunocytochemistry and MMP-9 mRNA was expressed by bronchial epithelial cells of lung tissue section shown by in situ hybridisation. MMP-2 seems not to play a major role in chronic lung inflammation. No clear differences in MMP-2 or MMP-14 (a potent MMP-2 activator) levels were found when comparing the samples from COPD or healthy horses. These results suggests that MMP-9 could serve as a potential diagnostic marker for the active ongoing tissue remodelling in the acute phase of equine COPD. Increased gelatinolytic activity could be found at both tested respiratory tract levels. Therefore, tracheal epithelial lining fluid (TELF) samples can usefully serve as diagnostic material for detection of increased levels of the main gelatinolytic MMP, MMP-9, representing the entire diseased lung.
S M Raulo; T Sorsa; T Tervahartiala; E Pirilä; P Maisi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Equine veterinary journal     Volume:  33     ISSN:  0425-1644     ISO Abbreviation:  Equine Vet. J.     Publication Date:  2001 Mar 
Date Detail:
Created Date:  2001-03-26     Completed Date:  2001-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0173320     Medline TA:  Equine Vet J     Country:  England    
Other Details:
Languages:  eng     Pagination:  128-36     Citation Subset:  IM    
Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland.
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MeSH Terms
Biological Markers
Blotting, Western / veterinary
Bronchoalveolar Lavage Fluid / chemistry,  cytology
Case-Control Studies
Horse Diseases / diagnosis*,  enzymology,  immunology
Immunohistochemistry / veterinary
Inflammation / enzymology,  veterinary*
Lung Diseases, Obstructive / diagnosis,  enzymology,  immunology,  veterinary*
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism*
Trachea / cytology,  metabolism
Reg. No./Substance:
0/Biological Markers; EC Metalloproteinase 2; EC Metalloproteinase 9
Comment In:
Equine Vet J. 2001 Mar;33(2):113-5   [PMID:  11266058 ]

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