| MIR-210 modulates mitochondrial respiration in placenta with preeclampsia. | |
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MedLine Citation:
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PMID: 22840297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Preeclampsia (PE) affects 5-8% of all pregnancies and is associated with significant maternal and fetal morbidity and mortality. Placental mitochondrial dysfunction has been reported in PE. MicroRNAs (miRNA) are small non-coding RNAs that regulate gene expression through mRNA degradation and translational repression. MiR-210 has been previously shown to be upregulated in placentas from pregnancies complicated by PE. We hypothesized that placental mitochondrial dysfunction during PE can be mediated by miR-210. Placentas were collected at term from normotensive pregnancies (CTRL) and those complicated by severe PE (n = 6 each) following c-section (no labor). Villous tissue from PE showed significantly increased levels of HIF-1α compared to CTRL with no change in corresponding mRNA expression but with reduced DNA-binding activity. Mitochondrial complex III was significantly decreased in PE along with significantly reduced protein expression in complex I and IV during PE. Among the four miRNAs tested, miR-210 showed significant up regulation in PE and significant downregulation of its target, ISCU mRNA. To understand the role of miR-210 in PE, loss- and gain-of-function studies were performed using primary trophoblasts. Trophoblasts were transfected with miR-210 inhibitor or pre-miR-210 and mitochondrial function was measured using Seahorse Extracellular Flux Analyzer. Cells transfected with pre-miR-210 showed significant reduction in oxygen consumption. In contrast, transfection of trophoblast with AntagomiR-210 was sufficient to prevent the DFO-mediated respiratory deficiency. These data collectively suggest that miR-210 overexpression during PE could be responsible for placental mitochondria dysfunction. |
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Authors:
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S Muralimanoharan; A Maloyan; J Mele; C Guo; L G Myatt; L Myatt |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-07-26 |
Journal Detail:
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Title: Placenta Volume: 33 ISSN: 1532-3102 ISO Abbreviation: Placenta Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-11 Completed Date: 2013-01-25 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 816-23 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Center for Pregnancy and Newborn Research, Dept of Ob-Gyn, University of Texas Health Science Center, San Antonio, TX 78229, USA. muralimanoha@uthscsa.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Down-Regulation Electron Transport Complex I / metabolism Electron Transport Complex III / metabolism Electron Transport Complex IV / metabolism Female Humans Hypoxia-Inducible Factor 1, alpha Subunit / metabolism MicroRNAs / physiology* Mitochondria / metabolism* Placenta / physiopathology* Pre-Eclampsia / physiopathology* Pregnancy |
| Grant Support | |
ID/Acronym/Agency:
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HL075297/HL/NHLBI NIH HHS; R01 HL075297/HL/NHLBI NIH HHS; UL1RR025767/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/MIRN210 microRNA, human; 0/MicroRNAs; EC 1.10.2.2/Electron Transport Complex III; EC 1.6.5.3/Electron Transport Complex I; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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