Document Detail


MIB-1 and p53 immunocytochemistry for differentiating pilocytic astrocytomas and astrocytomas from anaplastic astrocytomas and glioblastomas in children and young adults.
MedLine Citation:
PMID:  9839169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To establish whether MIB-1 and p53 staining are useful for differentiating pilocytic astrocytomas and astrocytomas from anaplastic astrocytomas and glioblastomas. This study was restricted to children and young adults under 30 years of age because of the differences in p53 mutations between paediatric and adult astrocytomas. METHODS AND RESULTS: Forty-five astrocytic tumours, including 18 pilocytic astrocytomas, 14 astrocytomas, four anaplastic astrocytomas and nine glioblastomas, from 45 children and young adults, between 1 and 29 years (mean 11 years) of age, were examined pathologically, and sections from paraffin-embedded blocks were used for MIB-1 and p53 immunostaining. The MIB-1 labelling index and the frequency and intensity of p53 staining in both the pilocytic astrocytoma and the astrocytoma group were significantly lower than in the anaplastic astrocytoma plus glioblastoma group (P < 0.001). In 11.1% (two of 18) of pilocytic astrocytomas and 42.9% (six of 14) of astrocytomas, immunoreactivity of either MIB-1 or p53 staining was of almost the same intensity as that of anaplastic astrocytomas and glioblastomas. However, in these cases, results using both MIB-1 and p53 stain differed from those for anaplastic astrocytomas and glioblastomas. CONCLUSIONS: MIB-1 and p53 co-staining is very useful for differentiating pilocytic astrocytomas and astrocytomas from anaplastic astrocytomas and glioblastomas. However, MIB-1 or p53 staining alone cannot differentiate pilocytic astrocytomas and astrocytomas from anaplastic astrocytomas and glioblastomas.
Authors:
T Matsumoto; T Fujii; M Yabe; K Oka; T Hoshi; K Sato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histopathology     Volume:  33     ISSN:  0309-0167     ISO Abbreviation:  Histopathology     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-02-03     Completed Date:  1999-02-03     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  446-52     Citation Subset:  IM    
Affiliation:
First Department of Pathology, Juntendo University, School of Medicine, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Antigens, Nuclear
Astrocytoma / chemistry,  genetics,  pathology*
Biological Markers / analysis
Child
Child, Preschool
Diagnosis, Differential
Female
Glioblastoma / chemistry,  genetics,  pathology*
Humans
Immunohistochemistry
Infant
Ki-67 Antigen
Male
Nuclear Proteins / analysis*
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Tumor Suppressor Protein p53 / analysis*,  genetics
Chemical
Reg. No./Substance:
0/Antigens, Nuclear; 0/Biological Markers; 0/Ki-67 Antigen; 0/Nuclear Proteins; 0/Tumor Suppressor Protein p53

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