Document Detail

MHC class I dimer formation by alteration of the cellular redox environment and induction of apoptosis.
MedLine Citation:
PMID:  22044191     Owner:  NLM     Status:  MEDLINE    
Many MHC class I molecules contain unpaired cysteine residues in their cytoplasmic tail domains, the function of which remains relatively uncharacterized. Recently, it has been shown that in the small secretory vesicles known as exosomes, fully folded MHC class I dimers can form through a disulphide bond between the cytoplasmic tail domain cysteines, induced by the low levels of glutathione in these extracellular vesicles. Here we address whether similar MHC class I dimers form in whole cells by alteration of the redox environment. Treatment of the HLA-B27-expressing Epstein-Barr virus-transformed B-cell line Jesthom, and the leukaemic T-cell line CEM transfected with HLA-B27 with the strong oxidant diamide, and the apoptosis-inducing and glutathione-depleting agents hydrogen peroxide and thimerosal, induced MHC class I dimers. Furthermore, induction of apoptosis by cross-linking FasR/CD95 on CEM cells with monoclonal antibody CH-11 also induced MHC class I dimers. As with exosomal MHC class I dimers, the formation of these structures on cells is controlled by the cysteine at position 325 in the cytoplasmic tail domain of HLA-B27. Therefore, the redox environment of cells intimately controls induction of MHC class I dimers, the formation of which may provide novel structures for recognition by the immune system.
Dinara Makhadiyeva; Lorraine Lam; Mohammad Moatari; Jasmine Vallance; Ying Zheng; Elaine C Campbell; Simon J Powis
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  135     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-12     Completed Date:  2012-03-30     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  133-9     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Immunology © 2012 Blackwell Publishing Ltd.
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MeSH Terms
Antigens, CD95 / immunology
Apoptosis / drug effects,  immunology*
Cell Line
Histocompatibility Antigens Class I / drug effects,  immunology*
Hydrogen Peroxide / pharmacology
Thimerosal / pharmacology
Grant Support
ETM/56//Chief Scientist Office; //Chief Scientist Office
Reg. No./Substance:
0/Antigens, CD95; 0/Histocompatibility Antigens Class I; 2225PI3MOV/Thimerosal; BBX060AN9V/Hydrogen Peroxide

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