Document Detail

The MHC class II region and resistance to an intestinal parasite in sheep.
MedLine Citation:
PMID:  21395744     Owner:  NLM     Status:  PubMed-not-MEDLINE    
RFLP analysis was used to investigate the effect of genetic variation in the Major Histocompatibility Complex (MHC) class II region on resistance of sheep to the intestinal parasite Trichostrongylus colubriformis, using faecal egg count (fec) as the measure of resistance. RFLP analysis of DNA from 335 sheep with the restriction enzymes TaqI, PvuII and HindIII, and DRB, DQA and DQB human class II MHC cDNA clones, revealed 238 bands, of which 233 were polymorphic. Sixteen bands were associated with a significant effect on fec, when analysed using a mixed model, best linear unbiased prediction statistical methods. However, when p values were corrected for the number of bands tested the associations were no longer significant. While no individual band had a significant effect on fec, Fisher's X(2) test of the distribution of p-values showed that RFLP bands, when considered together, do account for a significant proportion of variation in fec. One hundred and thirteen offspring from 11 halb-sib families were classified according to which MHC haplotype they had inherited from their sire. While there was overall no convincing statistical evidence of an effect of MHC haplotype on resistance, results in one family showed evidence for a repeatable MHC effect. ZUSAMMENFASSUNG: Die MHC-Klasse II Region und Resistenz gegenüber Darmparasiten beim Schaf RFLP Analyse betrifft die Auswirkung genetischer Unterschiede im Haupthistokompatibilitäts-Komplex (MHC) Klasse II Region auf Resistenz von Schafen gegen den Darmparasiten Trichostrongylus colubriformis, wobei die fäkale Eizahl (fec) als Maß der Resistenz verwendet worden ist. Die RFLP-Analyse der DNA von 335 Schafen mit Restriktionsenzymen TaqI, PvuII und HndIII und DRB, DQA und DQB menschlicher Klasse II MHC cDNA Klone ergab 238 Bande, wovon 233 polymorph waren. 16 Bande waren mit einer signifikanten Wirkung auf fec verbunden, wobei zur Analyse das gemischte Modell der BLUP verwendet worden ist. Allerdings, nach Korrektur der p-Werte für die Zahl der geprüften Bande zeigt sich die Assoziation nicht länger als signifikant. Während kein individuelles Band eine signifikante Wirkung auf FEC zeigte, ergab Fisher's test hinsichtlich Verteilung der p-Werte Signifikanz bei Gesamtbetrachtung. 113 Nachkommen von 11 Halbgeschwister-Familien wurden nach dem MHC Haplotyp des Vatertieres klassifiziert. Es ergab sich kein insgesamt überzeugender statistischer Hinweis auf Wirkung der MHC Haplotypen, doch in einer Familie Hinweis auf wiederholbare MHC Effekte.
D J Hulme; F W Nicholas; R G Windon; S C Brown; K J Beh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of animal breeding and genetics = Zeitschrift für Tierzüchtung und Züchtungsbiologie     Volume:  110     ISSN:  0931-2668     ISO Abbreviation:  J. Anim. Breed. Genet.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  2011-03-14     Completed Date:  2012-10-02     Revised Date:  2013-05-20    
Medline Journal Info:
Nlm Unique ID:  100955807     Medline TA:  J Anim Breed Genet     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  459-72     Citation Subset:  -    
Copyright Information:
1993 Blackwell Verlag GmbH.
McMaster Laboratory, CSIRO Division of Animal Health, Private Bag 1, Glebe, NSW, Australia. Dept. Animal Science, University of Sydney, NSW, Australia. Pastoral Research Laboratory, CSIRO Division of Animal Health, Armidale, NSW, Australia.
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