| MEX-5 enrichment in the C. elegans early embryo mediated by differential diffusion. | |
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MedLine Citation:
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PMID: 20627961 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Specification of germline and somatic cell lineages in C. elegans originates in the polarized single-cell zygote. Several cell-fate determinants are partitioned unequally along the anterior-posterior axis of the zygote, ensuring the daughter cells a unique inheritance upon asymmetric cell division. Recent studies have revealed that partitioning of the germline determinant PIE-1 and the somatic determinant MEX-5 involve protein redistribution accompanied by spatiotemporal changes in protein diffusion rates. Here, we characterize the dynamics of MEX-5 in the zygote and propose a novel reaction/diffusion model to explain both its anterior enrichment and its remarkable intracellular dynamics without requiring asymmetrically distributed binding sites. We propose that asymmetric cortically localized PAR proteins mediate the anterior enrichment of MEX-5 by reversibly changing its diffusion rate at spatially distinct points in the embryo, thus generating a stable concentration gradient along the anterior-posterior axis of the cell. This work extends the scope of reaction/diffusion models to include not only germline morphogens, but also somatic determinants. |
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Authors:
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Brian R Daniels; Terrence M Dobrowsky; Edward M Perkins; Sean X Sun; Denis Wirtz |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 137 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-14 Completed Date: 2010-08-24 Revised Date: 2011-08-03 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 2579-85 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / metabolism, physiology* Cytoplasm / metabolism Diffusion Embryo, Nonmammalian / metabolism* Gene Expression Regulation, Developmental* Genes, Helminth Green Fluorescent Proteins / metabolism Microscopy, Fluorescence / methods Models, Biological Software Zygote / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA143868/CA/NCI NIH HHS; GM075305/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Caenorhabditis elegans Proteins; 0/MEX-5 protein, C elegans; 147336-22-9/Green Fluorescent Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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